Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma

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dc.contributor.authorUtkusavas, Ayfer
dc.contributor.authorGurel Gurevin, Ebru
dc.contributor.authorYilmazer, Nadim
dc.contributor.authorUvez, Ayca
dc.contributor.authorOztay, Fusun
dc.contributor.authorBulut, Huri
dc.date.accessioned2022-03-09T09:09:35Z
dc.date.available2022-03-09T09:09:35Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractCombined use of a chemotherapeutic agent and an autophagy inhibitor is a novel cancer treatment strategy. We investigated the effects of chloroquine (CQ) on lung pathology caused by both solid Ehrlich ascites carcinoma (EAC) and doxorubicin (DXR). A control group and eight experimental groups of adult female mice were inoculated subcutaneously with 2.5 × 106 EAC cells. DXR (1.5 mg/kg and 3 mg/kg) and CQ (25 mg/kg and 50 mg/kg) alone or in combination were injected intraperitoneally on days 2, 7 and 12 following inoculation with EAC cells. Lung tissue samples were examined using immunohistochemistry (IHC) for endothelial (eNOS), inducible nitric oxide synthase (iNOS) and neutrophil gelatinase-associated lipocalin (NGAL). Serum catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured using ELISA. We found decreased levels of iNOS and eNOS in the groups that received 1.5 mg/kg DXR alone and in combination with 25 mg/kg and 50 mg/kg CQ. Combined administration of DXR and CQ partially prevented disruption of alveolar structure. Levels of antioxidant enzymes and MDA were lower in all treated groups; the greatest reduction was observed in mice that received the combination of 25 mg/kg CQ + 1.5 mg/kg DXR. Levels of NGAL were elevated in all treated groups. We found that CQ ameliorated both EAC and DOX induced lung pathology in female mice with solid EAC by reducing oxidative stress.en_US
dc.identifier.citationUtkusavas A, Gurel Gurevin E, Yilmazer N, Uvez A, Oztay F, Bulut H, Ustunova S, Esener OBB, Sonmez K, Erol Kutucu D, Meral I, Dimas K, Armutak EI. Effects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinoma. Biotech Histochem. 2022 Mar 4:1-12.en_US
dc.identifier.doi10.1080/10520295.2022.2036369en_US
dc.identifier.endpage12en_US
dc.identifier.pmid35240890en_US
dc.identifier.scopus2-s2.0-85126021989en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1080/10520295.2022.2036369
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2538
dc.identifier.wosWOS:000764954500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBulut, Huri
dc.language.isoenen_US
dc.relation.ispartofBiotech Histochemen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChloroquineen_US
dc.subjectEhrlich Scites Carcinomaen_US
dc.subjectDoxorubicinen_US
dc.subjectLungen_US
dc.subjectMiceen_US
dc.subjectOxidative Stressen_US
dc.titleEffects of combined administration of doxorubicin and chloroquine on lung pathology in mice with solid Ehrlich ascites carcinomaen_US
dc.typeArticleen_US

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