A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens

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dc.authoridÖzlem Büyüktanır Yaş / 0000-0002-7641-7350en_US
dc.authorscopusidÖzlem Büyüktanır Yaş / 57221162761
dc.authorwosidÖzlem Büyüktanır Yaş / AAP-2720-2020
dc.contributor.authorYang, Xiuli
dc.contributor.authorKoci, Juraj
dc.contributor.authorSmith, Alexis A
dc.contributor.authorZhuang, Xuran
dc.contributor.authorSharma, Kavita
dc.contributor.authorDutta, Shraboni
dc.contributor.authorRana, Vipin Singh
dc.contributor.authorKitsou, Chrysoula
dc.contributor.authorBüyüktanır Yaş, Özlem
dc.contributor.authorMongodin, Emmanuel F
dc.contributor.authorPal, Utpal
dc.date.accessioned2020-10-02T13:03:24Z
dc.date.available2020-10-02T13:03:24Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractThe peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen. The structure is thought to play an important role in tick biology. The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance. Herein, we characterize a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix. The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium. The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance. Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression. The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced Borrelia burgdorferi acquisition in ticks and its transmission to mice. Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections. This article is protected by copyright. All rights reserved.en_US
dc.identifier.citationYang, X., Koči, J., Smith, A. A., Zhuang, X., Sharma, K., Dutta, S., Rana, V. S., Kitsou, C., Yas, O. B., Mongodin, E. F., & Pal, U. (2020). A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens. Cellular microbiology, e13275.en_US
dc.identifier.doi10.1111/cmi.13275en_US
dc.identifier.pmid33006213en_US
dc.identifier.scopus2-s2.0-85092790914en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1111/cmi.13275
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1130
dc.identifier.wosWOS:000579839300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBüyüktanır Yaş, Özlem
dc.language.isoenen_US
dc.relation.ispartofCellular microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogensen_US
dc.typeArticleen_US

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