Enhancing effect of nicotine on electrical field stimulation elicited contractile responses in isolated rabbit bladder straight muscle; the role of cannabinoid and vanilloid receptors

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dc.authoridYusuf Sarıoğlu / 0000-0002-9227-365Xen_US
dc.authorscopusidYusuf Sarıoğlu / 7003713314en_US
dc.authorwosidYusuf Sarıoğlu / DRN-8389-2022en_US
dc.contributor.authorİlhan, Sevil Özger
dc.contributor.authorFincan, Gökçe Sevim Öztürk
dc.contributor.authorOkçay, Yağmur
dc.contributor.authorKoç, Derya Sebile
dc.contributor.authorAşkin, Celil İlker
dc.contributor.authorKibar, Ayşe Kübra
dc.contributor.authorVural, İsmail Mert
dc.contributor.authorSarıoğlu, Yusuf
dc.date.accessioned2023-01-26T11:23:38Z
dc.date.available2023-01-26T11:23:38Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground/aim: Nicotine acts as an agonist of nicotinic acetylcholine receptors (nAChR). These receptors belong to a superfamily of ligand-gated ion channels. We previously demonstrated that nicotine increased electrical field stimulation (EFS)-induced contractile or relaxation responses, possibly by facilitating neurotransmitter release from nerve terminals in various rabbit tissues. Studies have shown that there is an interaction between the endocannabinoid and nicotinic systems. This study aimed to investigate the interaction between nicotine and the endocannabinoid system in the rabbit urine bladder and also investigate the enhancing effect of nicotine on EFS-induced contractile responses in rabbit isolated bladder smooth muscle and its interaction with the endocannabinoid system. Materials and methods: The New Zealand albino male adult rabbits were used for this study. Following scarification, the urine bladder was rapidly excised, and then uniform strips were prepared. Each strip was mounted under 1 g isometric resting tension in an organ bath containing 20 mL of Krebs–Henseleit solution. After obtaining EFS-induced contractile responses, 10–4 M concentrations of nicotine were applied to the preparations, and EFS was stopped after 5 stimulations. Following washing, the same experimental procedure was performed with the same tissue in the presence of AM251 (a cannabinoid CB1R antagonist, 10–6 M), AM630 (a cannabinoid CB2R antagonist, 10–6 M), and capsazepine (a vanilloid receptor antagonist, 3 × 10–6 M). Results: Nicotine enhanced the EFS-induced contraction responses by 17.16% ± 2.81% at a 4-Hz stimulation frequency. Cannabinoid receptor antagonists AM251 and AM630 reduced this increasing effect of nicotine although it was not significant and vanilloid receptor antagonist capsazepine did not significantly alter the nicotines’ effect. Conclusion: These results show that enhancing effect of nicotine in the smooth muscle of the rabbit bladder, even though it was not significant endocannabinoid system possibly have a role in nicotines’ effect. © TÜBİTAK.en_US
dc.identifier.citationİLHAN, S. Ö., FİNCAN, G. S. Ö., OKÇAY, Y., KOÇ, D. S., AŞKIN, C. İ., KİBAR, A. K., ... & SARIOĞLU, Y. (2022). Enhancing effect of nicotine on electrical field stimulation elicited contractile responses in isolated rabbit bladder straight muscle; the role of cannabinoid and vanilloid receptors. Turkish Journal of Medical Sciences, 52(6), 1814-1820.en_US
dc.identifier.doi10.55730/1300-0144.5527en_US
dc.identifier.endpage1820en_US
dc.identifier.issn1300-0144en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85144558021en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1814en_US
dc.identifier.trdizinid1145531en_US
dc.identifier.urihttp://doi.org/10.55730/1300-0144.5527
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3835
dc.identifier.volume52en_US
dc.identifier.wosWOS:000906716200009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorSarıoğlu, Yusuf
dc.language.isoenen_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBladderen_US
dc.subjectCannabinoid Receptoren_US
dc.subjectElectrical Field Stimulationen_US
dc.subjectNicotineen_US
dc.subjectVanilloid Receptoren_US
dc.titleEnhancing effect of nicotine on electrical field stimulation elicited contractile responses in isolated rabbit bladder straight muscle; the role of cannabinoid and vanilloid receptorsen_US
dc.typeArticleen_US

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