Malabaricones from the fruit of Myristica cinnamomea King as potential agents against Acanthamoeba castellanii

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dc.authoridAWANG, KHALIJAH/0000-0001-5971-6570
dc.authoridOthman, Muhamad Aqmal/0000-0001-5309-3253
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridKhan, khalid/0000-0001-8337-4021
dc.authorwosidKhan, Khalid M/W-4752-2018
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidAWANG, KHALIJAH/B-8781-2010
dc.authorwosidOthman, Muhamad Aqmal/AAA-3999-2020
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.contributor.authorAhmed, Usman
dc.contributor.authorSivasothy, Yasodha
dc.contributor.authorKhan, Khalid Mohammed
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorWahab, Siti Mariam Abdul
dc.contributor.authorAwang, Khalijah
dc.contributor.authorOthman, Muhamad Aqmal
dc.date.accessioned2024-05-19T14:38:47Z
dc.date.available2024-05-19T14:38:47Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAcanthamoeba castellanii is an opportunistic free-living amoeba (FLA) pathogen which can cause fatal central nervous system (CNS) infection, granulomatous amoebic encephalitis (GAE) and potentially blinding ocular infection, Acanthamoeba keratitis (AK). Acanthamoeba species remain a challenging protist to treat due to the unavailability of safe and effective therapeutic drugs and their ability to protect themselves in the cyst stage. Natural products and their secondary metabolites play a pivotal role in drug discovery against various pathogenic microorganisms. In the present study, the ethyl acetate extract of Myristica cinnamomea King fruit was evaluated against A. castellanii (ATCC 50492), showing an IC50 of 45.102 +/- 4.62 mu g/mL. Previously, the bio-guided fractionation of the extract resulted in the identification of three active compounds, namely Malabaricones (A-C). The isolated and thoroughly characterized acylphenols were evaluated for their anti-amoebic activity against A. castellanii for the first time. Among tested compounds, Malabaricone B (IC50 of 101.31 +/- 17.41 mu M) and Malabaricone C (IC50 of 49.95 +/- 6.33 mu M) showed potent anti-amoebic activity against A. castellanii trophozoites and reduced their viability up-to 75 and 80 %, respectively. Moreover, both extract and Malabaricones also significantly (p < 0.05) inhibit the encystation and excystation of A. castellanii, while showed minimal toxicity against human keratinocyte cells (HaCaT cells) at lower tested concentrations. Following that, the explanation of the possible mechanism of action of purified compounds were assessed by detection of the state of chromatin. Hoechst/PI 33342 double staining showed that necrotic cell death occurred in A. castellanii trophozoites after 8 h treatment of Malabaricones (A-C). These findings demonstrate that Malabaricones B and C could serve as promising therapeutic options against A. castellanii infections.en_US
dc.description.sponsorshipSunway University, Malaysia [GRTIN-IRG-10-2022]en_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia (Individual research grant, GRTIN-IRG-10-2022) .en_US
dc.identifier.doi10.1016/j.actatropica.2023.107033
dc.identifier.issn0001-706X
dc.identifier.issn1873-6254
dc.identifier.pmid37783284en_US
dc.identifier.scopus2-s2.0-85174333029en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1016/j.actatropica.2023.107033
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4605
dc.identifier.volume248en_US
dc.identifier.wosWOS:001098024000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofActa Tropicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcanthamoebaen_US
dc.subjectMyristica Cinnamomea Kingen_US
dc.subjectAcylphenolsen_US
dc.subjectMalabaricones A-Cen_US
dc.titleMalabaricones from the fruit of Myristica cinnamomea King as potential agents against Acanthamoeba castellaniien_US
dc.typeArticleen_US

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