IgE and IgG4 binding to lentil epitopes in children with red and green lentil allergy

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dc.authoridBaran Erman / 0000-0001-9398-8465
dc.authorscopusidBaran Erman / 55214373300
dc.authorwosidBaran Erman / G-1409-2015
dc.contributor.authorSaçkesen, Cansın
dc.contributor.authorErman, Baran
dc.contributor.authorGimenez, Gustavo
dc.contributor.authorGrishina, Galina
dc.contributor.authorYılmaz, Özlem
dc.contributor.authorYavuz, Süleyman T.
dc.contributor.authorŞahiner, Ümit M.
dc.contributor.authorBüyüktiryaki, Betül
dc.contributor.authorYılmaz, Ebru A.
dc.contributor.authorÇavkaytar, Özlem
dc.contributor.authorSampson, Hugh A.
dc.date.accessioned2020-08-30T20:06:27Z
dc.date.available2020-08-30T20:06:27Z
dc.date.issued2020
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractBackground The consumption of lentil is common in the Mediterranean area and is one of the causes of IgE-mediated food allergy in many countries. Len c 1 is a well-defined allergen of lentil and approximately 80% of the patients with lentil allergy recognize the purified Len c 1 protein. We sought to identify IgE and IgG4 sequential epitopes of Len c 1 in patients with red and/or green lentil allergy. We also aimed to determine IgE and IgG4 binding differences between those patients who had outgrown or remained reactive to lentil. Methods Children with IgE-mediated lentil allergy were included in the study. We applied a microarray immunoassay to determine the characterization of positive IgE and IgG4 binding to Len c 1 epitopes in the patients' sera. Results The peptides specifically recognized by IgE and IgG4 antibodies were mainly detected between peptides 107 and 135 of Len c 1. The signal intensities of positive epitopes were significantly greater in reactive patients than tolerant ones (P = .008 for IgE and P = .002 for IgG4). Moreover, IgE and IgG4 antibodies bound largely the same sequential epitopes in patients who remained reactive or outgrew their allergy. Conclusion IgG4-binding epitopes in lentil allergy were identified and IgE and IgG4 binding to epitopes in both red and green lentils was compared. Our data regarding signal intensity differences between reactive and outgrown patients and overlap binding of IgE and IgG4 antibodies may be important for the development of more accurate diagnostic tests and understanding of natural tolerance development.en_US
dc.description.sponsorshipDavid H. and Julia Koch Research Program in Food Allergy Therapeuticsen_US
dc.description.sponsorshipThis study was supported in part by the David H. and Julia Koch Research Program in Food Allergy Therapeutics.en_US
dc.identifier.citationSackesen, C., Erman, B., Gimenez, G., Grishina, G., Yilmaz, O., Yavuz, S. T., ... & Sampson, H. A. (2020). IgE and IgG4 binding to lentil epitopes in children with red and green lentil allergy. Pediatric Allergy and Immunology, 31(2), 158-166.en_US
dc.identifier.doi10.1111/pai.13136en_US
dc.identifier.endpage166en_US
dc.identifier.issn0905-6157en_US
dc.identifier.issn1399-3038en_US
dc.identifier.issue2en_US
dc.identifier.pmid31598994en_US
dc.identifier.scopus2-s2.0-85074774742en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage158en_US
dc.identifier.urihttps://doi.org/10.1111/pai.13136
dc.identifier.urihttps://hdl.handle.net/20.500.12713/521
dc.identifier.volume31en_US
dc.identifier.wosWOS:000493798700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErman, Baranen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofPediatric Allergy and Immunologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLen C 1en_US
dc.subjectLentil Allergyen_US
dc.subjectMicroarray-Based Immunoassayen_US
dc.subjectNatural Toleranceen_US
dc.titleIgE and IgG4 binding to lentil epitopes in children with red and green lentil allergyen_US
dc.typeArticleen_US

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