Association of clinical features with spike glycoprotein mutations in Iranian COVID-19 patients

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dc.authoridAtefeh Zarepour / 0000-0002-0347-5840en_US
dc.authoridAli Zarrabi / 0000-0003-0391-1769en_US
dc.authorscopusidAtefeh Zarepour / 56700291100en_US
dc.authorscopusidAli Zarrabi / 23483174100en_US
dc.authorwosidAtefeh Zarepour / AAH-9225-2020en_US
dc.authorwosidAli Zarrabi / U-2602-2019en_US
dc.contributor.authorAhangarzadeh, Shahrzad
dc.contributor.authorYousefi, Alireza
dc.contributor.authorRanjbar, Mohammad Mehdi
dc.contributor.authorDabiri, Arezou
dc.contributor.authorZarepour, Atefeh
dc.contributor.authorSadeghi, Mahmoud
dc.contributor.authorHeidari, Elham
dc.contributor.authorMazrui, Fariba
dc.contributor.authorHosseinzadeh, Majid
dc.contributor.authorAtaei, Behrooz
dc.contributor.authorZarrabi, Ali
dc.contributor.authorShariati, Laleh
dc.contributor.authorJavanmard, Shaghayegh Haghjooy
dc.date.accessioned2022-12-08T08:21:30Z
dc.date.available2022-12-08T08:21:30Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.description.abstractBackground: Mutations in spike glycoprotein, a critical protein of SARS-CoV-2, could directly impact pathogenicity and virulence. The D614G mutation, a non-synonymous mutation at position 614 of the spike glycoprotein, is a predominant variant circulating worldwide. This study investigated the occurrence of mutations in the crucial zone of the spike gene and the association of clinical symptoms with spike mutations in isolated viruses from Iranian patients infected with SARS-CoV-2 during the second and third waves of the COVID-19 epidemic in Isfahan, the third-largest city in Iran. Methods: The extracted RNA from 60 nasopharyngeal samples of COVID-19 patients were subjected to cDNA synthesis and RT-PCR (in three overlapping fragments). Each patient's reverse transcriptase polymerase chain reaction (RT-PCR) products were assembled and sequenced. Information and clinical features of all sixty patients were collected, summarized, and analyzed using the GENMOD procedure of SAS 9.4. Results: Analysis of 60 assembled sequences identified nine nonsynonymous mutations. The D614G mutation has the highest frequency among the amino acid changes. In our study, in 31 patients (51.66%), D614G mutation was determined. For all the studied symptoms, no significant relationship was observed with the incidence of D614G mutation. Conclusions: D614G, a common mutation among several of the variants of SARS-CoV-2, had the highest frequency among the studied sequences and its frequency increased significantly in the samples of the third wave compared to the samples of the second wave of the disease.en_US
dc.identifier.citationAhangarzadeh, S., Yousefi, A., Ranjbar, M. M., Dabiri, A., Zarepour, A., Sadeghi, M., ... & Haghjooy Javanmard, S. (2022). Association of Clinical Features with Spike Glycoprotein Mutations in Iranian COVID-19 Patients. Journal of Clinical Medicine, 11(21), 6315.en_US
dc.identifier.doi10.3390/jcm11216315en_US
dc.identifier.issn2077-0383en_US
dc.identifier.issue21en_US
dc.identifier.scopus2-s2.0-85141664701en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/jcm11216315
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3432
dc.identifier.volume11en_US
dc.identifier.wosWOS:000884119200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZarepour, Atefeh
dc.institutionauthorZarrabi, Ali
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofJOURNAL OF CLINICAL MEDICINEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCOVID-19en_US
dc.subjectClinical Symptomsen_US
dc.subjectSpike Glycoproteinen_US
dc.subjectMutationen_US
dc.subjectD614Gen_US
dc.titleAssociation of clinical features with spike glycoprotein mutations in Iranian COVID-19 patientsen_US
dc.typeArticleen_US

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