Effects of VEGF(+) mesenchymal stem cells and platelet-rich plasma on inbred rat ovarian functions in cyclophosphamide-induced premature ovarian insufficiency model

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorVural, Birol
dc.contributor.authorDuruksu, Gökhan
dc.contributor.authorVural, Fisun
dc.contributor.authorGörgüç, Merve
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-08-30T20:06:40Z
dc.date.available2020-08-30T20:06:40Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractPremature ovarian insufficiency (POI), a fertility disorder affecting women under 40years of age, is characterized by early loss of ovarian function. This study was aimed to maintain ovarian function in POI animal models by mesenchymal stem cells (MSCs) transplantation with/without the supplementation of platelet-rich plasma (PRP). Adipose tissue-derived MSCs were isolated from inbred rats (Fisher-344), and constitutive expression of both VEGF and GFP were maintained by transfection with plasmids, pVEGF and pGFP-N. PRP was derived from the blood of healthy untreated rats. A total of 60 rats were divided into 5 groups of 12 rats in each. First group was kept as untreated-control (Control), and POI model was induced in Fisher-344 rats by cyclophosphamide in the next four groups. Second group was kept as sham-operated-control (Sham). MSC, PRP and MSC+ PRP-treated groups were transplanted following the validation of POI model in rats. After 2months following the transplantation, anti-mullerian-hormone (AMH) and oestradiol (E-2) blood levels were measured. Follicles were evaluated after hematoxylin-eosin staining, and the immunofluorescence staining and gene expression analyses were performed to show the ovarian regeneration. The follicular count was improved after MSC- and MSC+PRP-treatment to 63% of Control-group and significantly higher than that in Sham-group, but a significant increase was not observed in PRP-group. Higher AMH and E-2 levels were measured in MSC+PRP than in Sham-group, and CXCL12, BMP-4, TGF-beta and IGF-1 expressions were also increased. This study showed MSCs +/-PRP transplantation after POI supports recovery of the follicular count and function. For ovarian recovery, a single administration of PRP was found not sufficient. Although MSC treatment increased follicular regeneration, better results were obtained in the co-transplantation of MSCs and PRP. These results might be promising for follicular regeneration in POI patients.en_US
dc.description.sponsorshipScientific and Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114S398]en_US
dc.description.sponsorshipWe thank Experimental Medicine Research and Application Unit (DETAB) at Kocaeli University for their assistance in our experimental work. This work was supported by the Scientific and Research Council of Turkey (TUBITAK) [grant number 114S398].en_US
dc.identifier.citationVural, B., Duruksu, G., Vural, F., Gorguc, M., & Karaoz, E. (2019). Effects of VEGF+ mesenchymal stem cells and platelet-rich plasma on inbred rat ovarian functions in cyclophosphamide-induced premature ovarian insufficiency model. Stem cell reviews and reports, 15(4), 558-573.en_US
dc.identifier.doi10.1007/s12015-019-09892-5en_US
dc.identifier.endpage573en_US
dc.identifier.issn1550-8943en_US
dc.identifier.issn1558-6804en_US
dc.identifier.issue4en_US
dc.identifier.pmid31037585en_US
dc.identifier.scopus2-s2.0-85065203803en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage558en_US
dc.identifier.urihttps://doi.org/10.1007/s12015-019-09892-5
dc.identifier.urihttps://hdl.handle.net/20.500.12713/588
dc.identifier.volume15en_US
dc.identifier.wosWOS:000475605700008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofStem Cell Reviews and Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCyclophosphamideen_US
dc.subjectGranulosa Cellsen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectOocyteen_US
dc.subjectPlatelet-Rich Plasmaen_US
dc.subjectPremature Ovarian Insufficiencyen_US
dc.subjectTheca Cellsen_US
dc.subjectVascular Endothelial Growth Factoren_US
dc.titleEffects of VEGF(+) mesenchymal stem cells and platelet-rich plasma on inbred rat ovarian functions in cyclophosphamide-induced premature ovarian insufficiency modelen_US
dc.typeArticleen_US

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