Influence of Metal Ions and Organic Solutions on Activity and Stability of Beta-Glucosidase Nanoflowers
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
beta-Glucosidase, a pivotal enzyme in the synthesis of various glycosides with wide applications in industries such as food, cosmetics, beverages, bioenergy, nutraceuticals, pharmaceuticals, and detergents, undergoes immobilization to enhance its utility in industrial processes and improve enzymatic properties. In this study, the conventional hybrid nanoflower synthesis method was used for beta-glucosidase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, the influence of metallic ions and various organic solvents on hybrid nanoflowers to enhance the activity and stability of the biocatalyst were investigated. The optimal morphology for beta-Glu/NFs was determined to be 0.01 mg/mL. To obtain the optimum activity conditions, we explored pH values ranging from 3 to 8 and temperatures between 30 and 80 degrees C. Both the free form and beta-Glu/NFs exhibited higher activity in acidic conditions, with a significant decrease in activity observed at pH 7 and 8. beta-Glu/NFs demonstrated superior activity compared to the free enzyme in the presence of metal ions, exhibiting a remarkable 3.6-fold increase in activity in chloroform, and approximately 2.8 and 2.7 times increase in benzene and glycerol, respectively. Moreover, it displayed over a 2-fold increase in activity in methanol, ethanol, isopropanol, n-butanol, acetone, and DMSO.
Açıklama
Anahtar Kelimeler
Beta-Glucosidase, Nanoflowers, Enzyme-Inorganic Hybrid Nanoflowers, Catalytic Activity
Kaynak
Chemistryselect
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
9
Sayı
6
