Influence of Metal Ions and Organic Solutions on Activity and Stability of Beta-Glucosidase Nanoflowers

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dc.authoridAltinkaynak, Cevahir/0000-0003-0082-8521
dc.contributor.authorAltinkaynak, Cevahir
dc.contributor.authorSamsa, Canan Gulmez
dc.contributor.authorEkremoglu, Murat
dc.contributor.authorTurk, Merve
dc.contributor.authorOzturkler, Melek
dc.contributor.authorOzdemir, Nalan
dc.contributor.authorAtakisi, Onur
dc.date.accessioned2024-05-19T14:39:20Z
dc.date.available2024-05-19T14:39:20Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractbeta-Glucosidase, a pivotal enzyme in the synthesis of various glycosides with wide applications in industries such as food, cosmetics, beverages, bioenergy, nutraceuticals, pharmaceuticals, and detergents, undergoes immobilization to enhance its utility in industrial processes and improve enzymatic properties. In this study, the conventional hybrid nanoflower synthesis method was used for beta-glucosidase-inorganic hybrid nanoflowers. Following a comprehensive pH and temperature scanning of the nanoflowers, the influence of metallic ions and various organic solvents on hybrid nanoflowers to enhance the activity and stability of the biocatalyst were investigated. The optimal morphology for beta-Glu/NFs was determined to be 0.01 mg/mL. To obtain the optimum activity conditions, we explored pH values ranging from 3 to 8 and temperatures between 30 and 80 degrees C. Both the free form and beta-Glu/NFs exhibited higher activity in acidic conditions, with a significant decrease in activity observed at pH 7 and 8. beta-Glu/NFs demonstrated superior activity compared to the free enzyme in the presence of metal ions, exhibiting a remarkable 3.6-fold increase in activity in chloroform, and approximately 2.8 and 2.7 times increase in benzene and glycerol, respectively. Moreover, it displayed over a 2-fold increase in activity in methanol, ethanol, isopropanol, n-butanol, acetone, and DMSO.en_US
dc.description.sponsorshipScientific Research Projects Committee of Nevsehir Haci Bektas Veli University; Mardin, Turkey; [BBAP20F14]en_US
dc.description.sponsorshipThis study is partially supported by Scientific Research Projects Committee of Nevsehir Haci Bektas Veli University (Project No: BBAP20F14). This study was partially presented in 8th International Mardin Artuklu Scientific Researchers Conference, June 4-6, 2022, Mardin, Turkey.en_US
dc.identifier.doi10.1002/slct.202304315
dc.identifier.issn2365-6549
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85184713299en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1002/slct.202304315
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4757
dc.identifier.volume9en_US
dc.identifier.wosWOS:001160809600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofChemistryselecten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBeta-Glucosidaseen_US
dc.subjectNanoflowersen_US
dc.subjectEnzyme-Inorganic Hybrid Nanoflowersen_US
dc.subjectCatalytic Activityen_US
dc.titleInfluence of Metal Ions and Organic Solutions on Activity and Stability of Beta-Glucosidase Nanoflowersen_US
dc.typeArticleen_US

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