Mesenchymal stem cells derived-exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation

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dc.contributor.authorErkoc-Biradli, Fatma Zehra
dc.contributor.authorErenay, Berkay
dc.contributor.authorOzgun, Alp
dc.contributor.authorOztatli, Hayriye
dc.contributor.authorIsik, Ferda
dc.contributor.authorAtes, Utku
dc.contributor.authorRasier, Rifat
dc.date.accessioned2024-05-19T14:38:58Z
dc.date.available2024-05-19T14:38:58Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAmniotic membrane extract (AME) and Wharton's jelly mesenchymal stem cells derived-exosomes (WJ-MSC-Exos) are promising therapeutic solutions explored for their potential in tissue engineering and regenerative medicine, particularly in skin and corneal wound healing applications. AME is an extract form of human amniotic membrane and known to contain a plethora of cytokines and growth factors, making it a highly attractive option for topical applications. Similarly, WJ-MSC-Exos have garnered significant interest for their wound healing properties. Although WJ-MSC-Exos and AME have been used separately for wound healing research, their combined synergistic effects have not been studied extensively. In this study, we evaluated the effects of both AME and WJ-MSC-Exos, individually and together, on the proliferation of corneal keratocytes as well as their ability to promote in vitro cell migration, wound healing, and their impact on cellular morphology. Our findings indicated that the presence of both exosomes (3 x 10(5) Exo/mL) and AME (50 mu g/mL) synergistically enhance the proliferation of corneal keratocytes. Combined use of these solutions (3 x 10(5) Exo/mL + 50 mu g/mL) increased cell proliferation compared to only 50 mu g/mL AME treatment on day 3 (**** p < 0.0001). This mixture treatment (3 x 10(5) Exo/mL + 50 mu g/mL) increased wound closure rate compared to isolated WJ-MSC-Exo treatment (3 x 10(5) Exo/mL) (*p < 0.05). Overall, corneal keratocytes treated with AME and WJ-MSC-Exo (3 x 10(5) Exo/mL + 50 mu g/mL) mixture resulted in enhanced proliferation and wound healing tendency. Utilization of combined use of AME and WJ-MSC-Exo can pave the way for a promising foundation for corneal repair research.en_US
dc.description.sponsorshipBogazici University Research Fund; Council of Higher Education for 100/2000 CoHE PhD Scholarship; Biomimetic and Bioinspired Biomaterials Research Laboratory; [6701]en_US
dc.description.sponsorshipThis study was partially supported by Bogazici University Research Fund by Grant Number 6701. F.Z.E.B would like to thank the Council of Higher Education for 100/2000 CoHE PhD Scholarship. F.Z.E.B and B.G would like to thank Joanne Anderson for the language editing and support to the Biomimetic and Bioinspired Biomaterials Research Laboratory. Graphical abstract has been created with .en_US
dc.identifier.doi10.1002/btpr.3465
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.pmid38602120en_US
dc.identifier.scopus2-s2.0-85190416316en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1002/btpr.3465
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4665
dc.identifier.wosWOS:001199938000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology Progressen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAmniotic Membrane Extracten_US
dc.subjectCorneal Keratocytesen_US
dc.subjectIn Vitro Corneal Wound Healingen_US
dc.subjectWharton's Jelly Mesenchymal Stem Cells Derived-Exosomesen_US
dc.titleMesenchymal stem cells derived-exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferationen_US
dc.typeArticleen_US

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