Self-assembled micelles loaded with itraconazole as anti-Acanthamoeba nano-formulation

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dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.contributor.authorRao, Komal
dc.contributor.authorAbdullah, Muhammad
dc.contributor.authorAhmed, Usman
dc.contributor.authorWehelie, Hashi Isse
dc.contributor.authorShah, Muhammad Raza
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorKhan, Naveed A.
dc.date.accessioned2024-05-19T14:45:48Z
dc.date.available2024-05-19T14:45:48Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAcanthamoeba castellanii are opportunistic pathogens known to cause infection of the central nervous system termed: granulomatous amoebic encephalitis, that mostly effects immunocompromised individuals, and a sight threatening keratitis, known as Acanthamoeba keratitis, which mostly affects contact lens wearers. The current treatment available is problematic, and is toxic. Herein, an amphiphilic star polymer with AB(2) miktoarms [A = hydrophobic poly(-Caprolacton) and B = hydrophilic poly (ethylene glycol)] was synthesized by ring opening polymerization and Cu-I catalyzed azide-alkyne cycloaddition. Characterization by H-1 and C-13 NMR spectroscopy, size-exclusion chromatography and fluorescence spectroscopy was accomplished. The hydrophobic drug itraconazole (ITZ) was incorporated in self-assembled micellar structure of AB(2) miktoarms through co-solvent evaporation. The properties of ITZ loaded (ITZ-PCL-PEG(2)) and blank micelles (PCL-PEG(2)) were investigated through zeta sizer, scanning electron microscopy and Fourier-transform infrared spectroscopy. Itraconazole alone (ITZ), polymer (DPB-PCL), empty polymeric micelles (PCL-PEG(2)) alone, and itraconazole loaded in polymeric micelles (ITZ-PCL-PEG(2)) were tested for anti-amoebic potential against Acanthamoeba, and the cytotoxicity on human cells were determined. The polymer was able to self-assemble in aqueous conditions and exhibited low value for critical micelle concentration (CMC) 0.05-0.06 mu g/mL. The maximum entrapment efficiency of ITZ was 68%. Of note, ITZ, DPB, PCL-PEG(2) and ITZ-PCL-PEG(2) inhibited amoebae trophozoites by 37.34%, 36.30%, 35.77%, and 68.24%, respectively, as compared to controls. Moreover, ITZ-PCL-PEG(2) revealed limited cytotoxicity against human keratinocyte cells. These results are indicative that ITZ-PCL-PEG(2) micelle show significantly better anti-amoebic effects as compared to ITZ alone and thus should be investigated further in vivo to determine its clinical potential.en_US
dc.description.sponsorshipAir Force Office of Scientific Research; Sunway University, Malaysia; University of Karachi, Pakistanen_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia, and University of Karachi, Pakistan.en_US
dc.identifier.doi10.1007/s00203-024-03854-3
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue4en_US
dc.identifier.pmid38433145en_US
dc.identifier.scopus2-s2.0-85186422137en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1007/s00203-024-03854-3
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5354
dc.identifier.volume206en_US
dc.identifier.wosWOS:001175936200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectMicellesen_US
dc.subjectItraconazoleen_US
dc.subjectAcanthamoebaen_US
dc.subjectNanoformulationen_US
dc.subjectCentral Nervous Systemen_US
dc.subjectInfectious Diseasesen_US
dc.subjectFree-Living Amoebaeen_US
dc.subjectNanotechnologyen_US
dc.titleSelf-assembled micelles loaded with itraconazole as anti-Acanthamoeba nano-formulationen_US
dc.typeArticleen_US

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