Selected Gut Bacteria from Water Monitor Lizard Exhibit Effects against Pathogenic Acanthamoeba castellanii Belonging to the T4 Genotype

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dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridAlharbi, Ahmad M./0000-0002-9512-1985
dc.authoridda Cruz Soares, Nelson/0000-0003-2331-8532
dc.authoridAkbar, Noor/0000-0002-8114-1969
dc.authoridGiddey, Alexander/0000-0002-2034-8122
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.contributor.authorAkbar, Noor
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorGiddey, Alexander D.
dc.contributor.authorSoares, Nelson C.
dc.contributor.authorAlharbi, Ahmad M.
dc.contributor.authorAlfahemi, Hasan
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.date.accessioned2024-05-19T14:42:04Z
dc.date.available2024-05-19T14:42:04Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractWater monitor lizards (WMLs) reside in unhygienic and challenging ecological surroundings and are routinely exposed to various pathogenic microorganisms. It is possible that their gut microbiota produces substances to counter microbial infections. Here we determine whether selected gut bacteria of water monitor lizards (WMLs) possess anti-amoebic properties using Acanthamoeba castellanii of the T4 genotype. Conditioned media (CM) were prepared from bacteria isolated from WML. The CM were tested using amoebicidal, adhesion, encystation, excystation, cell cytotoxicity and amoeba-mediated host cell cytotoxicity assays in vitro. Amoebicidal assays revealed that CM exhibited anti-amoebic effects. CM inhibited both excystation and encystation in A. castellanii. CM inhibited amoebae binding to and cytotoxicity of host cells. In contrast, CM alone showed limited toxic effects against human cells in vitro. Mass spectrometry revealed several antimicrobials, anticancer, neurotransmitters, anti-depressant and other metabolites with biological functions. Overall, these findings imply that bacteria from unusual places, such as WML gut, produce molecules with anti-acanthamoebic capabilities.en_US
dc.description.sponsorshipAir Force Office of Scientific Research (AFOSR) [FA 8655-20-1-7004]en_US
dc.description.sponsorshipThis work was funded, in part, by the Air Force Office of Scientific Research (AFOSR), grant number: FA 8655-20-1-7004.en_US
dc.identifier.doi10.3390/microorganisms11041072
dc.identifier.issn2076-2607
dc.identifier.issue4en_US
dc.identifier.pmid37110494en_US
dc.identifier.scopus2-s2.0-85156272962en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.3390/microorganisms11041072
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5195
dc.identifier.volume11en_US
dc.identifier.wosWOS:000979398400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMicroorganismsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectGut Microbiotaen_US
dc.subjectAcanthamoeba Castellaniien_US
dc.subjectWater Monitor Lizarden_US
dc.subjectCytopathogenicityen_US
dc.titleSelected Gut Bacteria from Water Monitor Lizard Exhibit Effects against Pathogenic Acanthamoeba castellanii Belonging to the T4 Genotypeen_US
dc.typeArticleen_US

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