Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut

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dc.authoridMaciver, Sutherland Kester/0000-0001-8234-6061
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridAkbar, Noor/0000-0002-8114-1969
dc.authoridAl-Hroub, Hamza/0000-0001-5621-9851
dc.authoridda Cruz Soares, Nelson/0000-0003-2331-8532
dc.authorwosidMaciver, Sutherland Kester/H-6284-2019
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAkbar, Noor
dc.contributor.authorSoares, Nelson Cruz
dc.contributor.authorAl-Hroub, Hamza Mohammad
dc.contributor.authorSemreen, Mohammad Harb
dc.contributor.authorMaciver, Sutherland K.
dc.contributor.authorKhan, Naveed Ahmed
dc.date.accessioned2024-05-19T14:39:02Z
dc.date.available2024-05-19T14:39:02Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPlain language summaryCrocodiles thrive in unsanitary conditions, feed on rotten meat, and endure conditions that are detrimental to human health. In addition to their immune system, we speculate that their microbial gut flora produce substances contributing to their hardiness and longevity. Herein, we showed that selected bacteria isolated from crocodile gut produced potent antibacterial properties against multiple drug-resistant pathogenic Gram-negative and Gram-positive bacteria. LC-MS/MS revealed the identity of gut microbial metabolites. These findings suggest that analyses of crocodile gut bacteria may reveal potential drug leads that can be utilized as probiotics/pre/post/antibiotics for the benefit of human health, however intensive future research is needed to realize these expectations. Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC-MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma-terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health. Tweetable abstractGut microbiome of hardy crocodile is a potential pharmacy that should be exploited for human benefit.en_US
dc.identifier.doi10.2144/fsoa-2023-0030
dc.identifier.issn2056-5623
dc.identifier.pmid37180607en_US
dc.identifier.scopus2-s2.0-85159333959en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.2144/fsoa-2023-0030
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4684
dc.identifier.wosWOS:000979093100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFuture Sci Ltden_US
dc.relation.ispartofFuture Science Oaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAntibiotic Resistanceen_US
dc.subjectGut Microbiotaen_US
dc.subjectMetabolomicsen_US
dc.titleMass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian guten_US
dc.typeArticleen_US

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