Fatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGE

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dc.authoridParham Taslimi / 0000-0002-3171-0633
dc.authorscopusidParham Taslimi / 56658628800
dc.authorwosidParham Taslimi / AAL-2788-2020
dc.contributor.authorErdoğan, Mehmet Kadir
dc.contributor.authorGeçibesler, İbrahim Halil
dc.contributor.authorYapar, Yakup
dc.contributor.authorGündoğdu, Ramazan
dc.contributor.authorKırıcı, Mahinur
dc.contributor.authorBehçet, Lütfi
dc.contributor.authorTaslimi, Parham
dc.date.accessioned2021-06-21T08:04:14Z
dc.date.available2021-06-21T08:04:14Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThis study attempts to evaluate the antioxidant, enzyme inhibitory, and anticancer properties as well as fatty acid compositions of endemic Saponaria prostrata WILLD. subsp. anatolica HEDGE. The gas chromatography-mass spectrometry (GC-MS) was used to determine the fatty acid content of methanol: dichloromethane extract from S. prostrata subsp. anatolica (SPA). Enzymatic activity was measured against acetylcholinesterase, butyrylcholinesterase and ?-glucosidase. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and Ferric reducing antioxidant power assay (FRAP) were conducted to antioxidant properties. The anticancer effect of SPA on human MCF-7 breast cancer and human HCT116 colorectal cancer cell line was evaluated by WST-1 cell viability assay, colony formation assay and wound healing assay. In addition, human VEGF Elisa method was used to determine the anti-angiogenic effect, and the quantitative real-time PCR (qRT-PCR) method on p53, Bax and Bcl-2 mRNA levels were used to evaluate apoptosis. While high amounts of palmitic acid (40.8%), linoleic acid (17.75%) and ?-linolenic acid (16.84%) were detected in the SPA, the total amount of unsaturated fatty acid (51.34%) was higher than the total amount of saturated fatty acid (48.66%). SPA displayed the most promising acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and ?-glycosidase (AG) inhibitory activities (AChE: IC50: 18.03 µg/mL, BuChE: IC50: 44.24 µg/mL and AG: IC50: 210.85 µg/mL). The half maximum inhibitory concentration (IC50) of SPA in MCF-7 and HCT116 cells was determined as 259.79 µg/mL and 97.24 µg/mL, respectively. In addition, it was determined that SPA suppresses colony formation and wound closure, and suppresses angiogenesis as well as triggering apoptosis at a significant level. It is true that endemic S. prostrata subsp. anatolica is a potential source of functional food ingredients, but more analytical and in vivo experiments are needed to explore further secondary metabolite diversity and pharmacological properties.en_US
dc.identifier.citationKadir Erdogan, M., Halil Gecibesler, I., Yapar, Y., Gundogdu, R., Kirici, M., Behcet, L., & Taslimi, P. (2021). Fatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGE. Bioorganic chemistry, 113, 105032. https://doi.org/10.1016/j.bioorg.2021.105032en_US
dc.identifier.doi10.1016/j.bioorg.2021.105032en_US
dc.identifier.endpage8en_US
dc.identifier.issue105032en_US
dc.identifier.pmid34089947en_US
dc.identifier.scopus2-s2.0-85108252731en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioorg.2021.105032
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1802
dc.identifier.volume113en_US
dc.identifier.wosWOS:000663792000003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTaslimi, Parham
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBioorganic Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticanceren_US
dc.subjectAntioxidanten_US
dc.subjectCaryophyllaceaeen_US
dc.subjectEndemic Planten_US
dc.subjectEnzyme Inhibitionen_US
dc.titleFatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGEen_US
dc.typeArticleen_US

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