Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon cancer

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dc.authoridHuri Bulut / 0000-0003-2706-9625en_US
dc.authorscopusidHuri Bulut / 57185264300
dc.authorwosidHuri Bulut / AAM-1432-2020
dc.contributor.authorGüleken, Zozan
dc.contributor.authorBulut, Huri
dc.contributor.authorGültekin, Güldal İnal
dc.contributor.authorArıkan, Soykan
dc.contributor.authorYaylım, İlhan
dc.contributor.authorHakan, Mehmet Tolgahan
dc.contributor.authorSönmez, Dilara
dc.contributor.authorTarhan, Nevzat K.
dc.contributor.authorDepciuch, Joanna
dc.date.accessioned2021-04-14T07:07:26Z
dc.date.available2021-04-14T07:07:26Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractColon and gastric cancers are the widespread benign types of cancers which are synchronous and metachronous neoplasms. In terms of the progression and progress of the disease, metabolic processes and differentiation in protein structures have an important role in for treatment of the disease. In this study we proposed to investigate the metabolic process and the differentiation of protein secondary structure among colon and gastric cancer as well as healthy controls using biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) methods. For this purpose, we measured blood serum of 133 patients, which were conducted upon oncology department (45 colon cancer, 45 gastric cancer and 43 control individuals). The obtained spectroscopic results and biochemical assays showed significant reduction in the amount of functional groups in cancer groups contrary with total protein measurements and structure of protein differences between colon and gastric cancers. Differentiations were visible in serum levels of CEA, CA-125, CA-15-3, CA-19-9 AFP (Alpha fetoprotein) of gastric and colon cancer patients as well as in amide III and secondly described amide I regions. Our findings suggest that amide I bonds in colon cancer cells can be helpful in diagnosis of colon cancer. Indeed, our results showed that metabolic processes were higher in gastric cancer group than in colon cancer. Hence, FTIR spectroscopy and curve-fitting analysis of amide I profile can be successfully applied as tools for identifying quantitative and qualitative changes of proteins in human cancerous blood serum. However, what is very important, in PCA analysis we see, that the scatter plot of PC1 (variability 80%) and PC2 (variability 15%) show that the data related to the control and two cancer groups are clustered together with different magnitudes and directions.en_US
dc.identifier.citationGuleken, Z., Bulut, H., Gültekin, G. İ., Arıkan, S., Yaylım, İ., Hakan, M. T., ... & Depciuch, J. (2021). Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon cancer. Talanta, 122353.en_US
dc.identifier.doi10.1016/j.talanta.2021.122353en_US
dc.identifier.issn0039-9140en_US
dc.identifier.pmid33965021en_US
dc.identifier.scopus2-s2.0-85103687562en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2021.122353
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1693
dc.identifier.volume231en_US
dc.identifier.wosWOS:000658306800013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBulut, Huri
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectColon Canceren_US
dc.subjectFTIRen_US
dc.subjectGastric Canceren_US
dc.subjectMetabolitesen_US
dc.subjectTotal Proteinen_US
dc.subjectTumor Markersen_US
dc.titleAssessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon canceren_US
dc.typeArticleen_US

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