Beneficial effects of adipose-derived stromal vascular fraction on testicular injury caused by busulfan

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dc.authorwosidElibol, Birsen/KFB-6195-2024
dc.contributor.authorHekimoglu, E. Rumeysa
dc.contributor.authorEsrefoglu, Mukaddes
dc.contributor.authorKarakaya Cimen, Fatma Bedia
dc.contributor.authorElibol, Birsen
dc.contributor.authorDedeakayogullari, Huri
dc.contributor.authorPasin, Ozge
dc.date.accessioned2024-05-19T14:39:43Z
dc.date.available2024-05-19T14:39:43Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe use of stem cells can attenuate testicular injury and promote sperm production. The adipose-derived stromal vascular fraction (SVF) has become an attractive cell source for cell-based therapies. In this study, we aimed to investigate the therapeutic efficacy of SVF on busulfan-induced testicular damage in rats. Twenty-four male rats were randomly divided into control, busulfan, SVF, and busulfan + SVF groups. Testicular damage was induced by intraperitoneal administration of busulfan (35 mg/kg). SVF obtained from human adipose tissue using Lipocube SVF (TM) was injected into rats 5 weeks after busulfan administration. At the end of the 8th week, rats were sacrificed, and histopathological, biochemical, and western blotting analyses were performed. No harmful effects of SVF on healthy testis tissue and sperm parameters were detected. SVF improved busulfan-induced oxidative stress in both testis tissue and serum. SVF injection to damaged testicular tissue resulted in increases in the healthy spermatozoon numbers and decreases in the abnormal tail numbers. Additionally, SVF increased bax/Bcl, DAZL, and TGF-beta 1 levels whereas decreased ATG5 and NF-kB levels. According to the results we obtained in this study, we suggest that SVF is beneficial in restoring damaged tissue by primarily being a multipotent cell source, by inhibiting oxidative stress and converting necrotic cell death to apoptotic cell death. In the future, clinical applications should bring higher benefits. Since SVF is the patient's own tissue, being harmless, it will offer an advantageous supportive treatment option for patients already weakened by cancer and anticancer therapy.en_US
dc.description.sponsorshipScientific Research Unit of Bezmialem Vakimath;f University (BAP) [20210825]en_US
dc.description.sponsorshipThe project was supported by the Scientific Research Unit of Bezmialem Vak & imath;f University (BAP No.: 20210825).en_US
dc.identifier.doi10.1080/01480545.2024.2324332
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.pmid38465409en_US
dc.identifier.scopus2-s2.0-85187447442en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1080/01480545.2024.2324332
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4834
dc.identifier.wosWOS:001182638800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofDrug and Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBusulfanen_US
dc.subjectStromal Vascular Fractionen_US
dc.subjectStem Cellen_US
dc.subjectInfertilityen_US
dc.subjectTesticular Damageen_US
dc.titleBeneficial effects of adipose-derived stromal vascular fraction on testicular injury caused by busulfanen_US
dc.typeArticleen_US

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