Display of Sukkula distributions on barley roots via in situ hybridization
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Firenze University Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Retrotransposon are an abundant and ancient parts of the plant genomes that especially LTR retrotransposons influence the genome size and evolution. Sukku-la is a non-autonomous and active, relatively high copy-number retroelement. In this study, we performed fluorescence in situ hybridization (FISH) to observe the distributions of Sukkula elements (LTRs and internal-domain) by using labelled-PCR products. The localizations of Sukkula elements (LTRs and internal-domain) were observed under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed that Sukkula elements is still active and spread through the whole barley chro-mosomes. Additionally, the re-sequencing analysis of Sukkula LTRs demonstrated that LTRs sequences had ~65 bp gain. These analyses represent a valuable resource to reveal genome organization of barley and large sized plants.
Açıklama
Anahtar Kelimeler
Barley, Fluorescence in Situ Hybridization, Internal-Domain, LTRs, Retrotransposon, Sukkula
Kaynak
Caryologia
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
N/A
Cilt
73
Sayı
3
Künye
Karlik, E. (2020). Display of Sukkula distributions on Barley Roots via in situ hybridization. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 73(3).
