Display of Sukkula distributions on barley roots via in situ hybridization

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dc.authoridElif Karlık / 0000-0003-0669-2725en_US
dc.authorscopusidElif Karlık / 56926577900
dc.authorwosidElif Karlık / N-6348-2018
dc.contributor.authorKarlık, Elif
dc.date.accessioned2021-05-17T09:39:16Z
dc.date.available2021-05-17T09:39:16Z
dc.date.issued2020en_US
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Eczane Hizmetleri Bölümüen_US
dc.description.abstractRetrotransposon are an abundant and ancient parts of the plant genomes that especially LTR retrotransposons influence the genome size and evolution. Sukku-la is a non-autonomous and active, relatively high copy-number retroelement. In this study, we performed fluorescence in situ hybridization (FISH) to observe the distributions of Sukkula elements (LTRs and internal-domain) by using labelled-PCR products. The localizations of Sukkula elements (LTRs and internal-domain) were observed under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed that Sukkula elements is still active and spread through the whole barley chro-mosomes. Additionally, the re-sequencing analysis of Sukkula LTRs demonstrated that LTRs sequences had ~65 bp gain. These analyses represent a valuable resource to reveal genome organization of barley and large sized plants.en_US
dc.identifier.citationKarlik, E. (2020). Display of Sukkula distributions on Barley Roots via in situ hybridization. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 73(3).en_US
dc.identifier.doi10.13128/caryologia-191en_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85105012264en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://www.doi.org/10.13128/caryologia-191
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1736
dc.identifier.volume73en_US
dc.identifier.wosWOS:000641027900009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarlık, Elif
dc.language.isoenen_US
dc.publisherFirenze University Pressen_US
dc.relation.ispartofCaryologiaen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBarleyen_US
dc.subjectFluorescence in Situ Hybridizationen_US
dc.subjectInternal-Domainen_US
dc.subjectLTRsen_US
dc.subjectRetrotransposonen_US
dc.subjectSukkulaen_US
dc.titleDisplay of Sukkula distributions on barley roots via in situ hybridizationen_US
dc.typeArticleen_US

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