High-fat diet enhances gastric contractility, but abolishes nesfatin-1-induced inhibition of gastric emptying

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dc.authoridAyça Karagöz Köroğlu / 0000-0002-2532-8091en_US
dc.authorscopusidAyça Karagöz Köroğlu / 57217538082
dc.authorwosidAyça Karagöz Köroğlu / DXI-1614-2022
dc.contributor.authorÖzdemir-Kumral, Zarife N.
dc.contributor.authorKoyuncuoğlu, Türkan
dc.contributor.authorArabacı-Tamer, Sevil
dc.contributor.authorÇilingir-Kaya, Özlem T.
dc.contributor.authorKaragöz Köroğlu, Ayça
dc.contributor.authorYüksel, Meral
dc.contributor.authorC. Yeğen, Berrak
dc.date.accessioned2021-04-05T09:05:05Z
dc.date.available2021-04-05T09:05:05Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground/Aims Gastrointestinal motility changes contribute to development and maintenance of obesity. Nesfatin-1 (NES-1) is involved in central appetite control. The aim is to elucidate effects of NES-1 and high-fat diet (HFD) on gastrointestinal motility and to explore myenteric neuron expressions of tyrosine hydroxylase (TH), vasoactive intestinal peptide (VIP), and neuronal nitric oxide synthase (nNOS) in HFDinduced oxidative injury. Methods Sprague-Dawley rats were fed with normal diet (ND) or HFD. Gastric emptying rate was measured following NES-1 (5 pmol/rat, intracerebroventricular) preceded by subcutaneous injections of glucagon-like peptide 1 (GLP-1), cholecystokinin 1 (CCK-1), and gastrin/CCK-2 receptor antagonists. In carbachol-contracted gastric and ileal strips, contractile changes were recorded by adding NES- 1 (0.3 nmol/L), GLP-1, CCK-1, and gastrin/CCK-2 antagonists. Results Neither HFD nor NES-1 changed methylcellulose emptying, but NES-1 delayed saline emptying in cannulated ND-rats. Inhibitory effect of NES-1 on gastric emptying in ND-rats was reversed by all antagonists, and abolished in HFD-rats. In HFD-rats, carbachol-induced contractility was enhanced in gastric, but inhibited in ileal strips. HFD increased body weight, while serum triglycerides, alanine transaminase, aspartate aminotransferase, glucose, and levels of malondialdehyde, glutathione, myeloperoxidase activity, and luminolchemiluminescence in hepatic, ileal, and adipose tissues were similar in ND- and HFD-rats, but only lucigenin-chemiluminescence was increased in HFD-rats. Vasoactive intestinal peptide (VIP) and TH immunoreactivities were depressed and nNOS immunoreactivity was increased in gastric tissues of HFD-rats, while VIP and TH were enhanced, but nNOS was reduced in their intestines. Conclusions HFD caused mild systemic inflammation, disrupted enteric innervation, enhanced gastric contractility, inhibited ileal contractility, and eliminated inhibitory effect of NES-1 on gastric motility.en_US
dc.identifier.citationÖzdemir-Kumral, Z. N., Koyuncuoğlu, T., Arabacı-Tamer, S., Çilingir-Kaya, Ö. T., Köroğlu, A. K., Yüksel, M., & Yeğen, B. Ç. (2021). High-fat Diet Enhances Gastric Contractility, but Abolishes Nesfatin-1-induced Inhibition of Gastric Emptying. Journal of neurogastroenterology and motility, 27(2), 265–278. https://doi.org/10.5056/jnm20206en_US
dc.identifier.doi10.5056/jnm20206en_US
dc.identifier.endpage278en_US
dc.identifier.issn2093-0879en_US
dc.identifier.issue2en_US
dc.identifier.pmid33795544en_US
dc.identifier.scopus2-s2.0-85104963384en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage265en_US
dc.identifier.urihttps://doi.org/10.5056/jnm20206
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1649
dc.identifier.volume27en_US
dc.identifier.wosWOS:000640313500001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaragöz Köroğlu, Ayça
dc.language.isoenen_US
dc.relation.ispartofJ Neurogastroenterol Motil.en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCholecystokininen_US
dc.subjectGastric Emptyingen_US
dc.subjectNesfatin-1en_US
dc.subjectNitric Oxide Synthase Type Ien_US
dc.subjectVasoactive Intestinal Peptideen_US
dc.titleHigh-fat diet enhances gastric contractility, but abolishes nesfatin-1-induced inhibition of gastric emptyingen_US
dc.typeArticleen_US

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