Search for novel plasma membrane proteins as potential biomarkers in human mesenchymal stem cells derived from dental pulp, adipose tissue, bone marrow, and hair follicle

dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorAkpınar, Gürler
dc.contributor.authorKaraosmanoğlu Yoneten, Kübra
dc.contributor.authorKasap, Murat
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2021-07-14T12:37:32Z
dc.date.available2021-07-14T12:37:32Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractOne of the drawbacks preventing the use of mesenchymal stem cells (MSCs) in clinical practice is the heterogeneous nature of their cultures. MSC cultures are not homogeneously formed by the MSCs and may contain non-mesenchymal cell types. Therefore, prior to use in clinics or research, complete characterization of MSCs should be performed to demonstrate the existence or absence of proper stem cell markers, many of which are happened to be cell-surface proteins. Unfortunately, the success of MSC characterization studies is limited due to the low specificity of the currently available cell-surface markers. Therefore, in this study, we aimed to investigate the plasma membrane (PM) proteins of MSCs isolated from human dental pulp (DP), adipose tissue (AT), bone marrow (BM), and hair follicle (HF) with the hope of proposing novel putative specific MSC markers. Differential-velocity centrifugation was used to enrich PM proteins. The isolated proteins were then identified by nLC-MS/MS and subjected to bioinformatics analysis. Proteins that were unique to each MSC type (CD9, CD10, CD63 for DP-MSCs; CD26, CD81, CD201, CD364 for AT-MSCs; Cd49a, CD49d for HF-MSCs; CD49e, CD56, CD92, CD97, CD156b, CD156c, CD220, CD221, CD298, CD315 for BM-MSCs) and common to all four MSC types (CD13, CD29, CD44, CD51, CD59, CD73, CD90) were identified. Uncharacterized proteins that have transmembrane (TM) domains were also detected. Some of the proteins identified in this study were the putative cell-surface markers that might be used for characterization of MSCs.en_US
dc.identifier.citationAkpinar, G., Yoneten, K. K., Kasap, M., & Karaoz, E. (2021). Search for Novel Plasma Membrane Proteins as Potential Biomarkers in Human Mesenchymal Stem Cells Derived from Dental Pulp, Adipose Tissue, Bone Marrow, and Hair Follicle. The Journal of membrane biology, 10.1007/s00232-021-00190-1. Advance online publication. https://doi.org/10.1007/s00232-021-00190-1en_US
dc.identifier.doi10.1007/s00232-021-00190-1en_US
dc.identifier.pmid34230997en_US
dc.identifier.scopus2-s2.0-85109339001en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1007/s00232-021-00190-1
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1927
dc.identifier.wosWOS:000670167600001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdal
dc.language.isoenen_US
dc.publisherSpringer Linken_US
dc.relation.ispartofThe Journal of Membrane Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCDsen_US
dc.subjectPlasma Membrane Proteomicsen_US
dc.subjectnLC-MS/MSen_US
dc.subjectMesenchymal Stem Cell Markersen_US
dc.titleSearch for novel plasma membrane proteins as potential biomarkers in human mesenchymal stem cells derived from dental pulp, adipose tissue, bone marrow, and hair follicleen_US
dc.typeArticleen_US

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