Toxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell lines

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dc.authoridNazlıhan Aztopal / 0000-0003-3118-8061en_US
dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidNazlıhan Aztopal / 55853882900
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidNazlıhan Aztopal / L-6687-2018
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorBalıkçı, Necmiye
dc.contributor.authorSarımahmut, Mehmet
dc.contributor.authorArı, Ferda
dc.contributor.authorAztopal, Nazlıhan
dc.contributor.authorÖzel, Mustafa Zafer
dc.contributor.authorUlukaya, Engin
dc.contributor.authorÇelikler, Serap
dc.date.accessioned2020-08-30T20:06:21Z
dc.date.available2020-08-30T20:06:21Z
dc.date.issued2020
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThere is a limited number of studies about the constituents of Hypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower and trunk were determined as eicosane, heptacosane, 2-propen-1-ol, hexahydrofarnesyl acetone and alpha-muurolene. HOE caused significant DNA damage at selected doses (250-750 mu g/ml) while chromosomal damage was observed at higher concentrations (500 and 750 mu g/ml). HOE demonstrated anti-growth activity in a dose-dependent manner between 3.13-100 mu g/ml. Pyknotic nuclei were observed at 100 mu g/ml concentration of HOE in both cell lines. In conclusion, HOE demonstrated cytotoxic effects in a cell type-dependent manner, however its genotoxic effects were observed at relatively higher doses.en_US
dc.description.sponsorshipBursa Uludag University [BUAP(F)-2014/3, OUAP(T)-2015/19]en_US
dc.description.sponsorshipYYYYYYY This work was supported by the Research Fund of Bursa Uludag University under Grants BUAP(F)-2014/3 and OUAP(T)-2015/19. The authors would like to thank Prof. Gurcan Guleryuz for his support in collection and identification of the plant and Ahmet Sari Mahmout for English editing and proofreading of the manuscript.en_US
dc.identifier.citationBalikci, N., Sarimahmut, M., Ari, F., Aztopal, N., Ozel, M. Z., Ulukaya, E., & Celikler, S. (2020). Toxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell lines. JOURNAL OF APPLIED BIOMEDICINE, 18(1), 18–25. https://doi.org/10.32725/jab.2020.002en_US
dc.identifier.doi10.32725/jab.2020.002en_US
dc.identifier.endpage25en_US
dc.identifier.issn1214-021Xen_US
dc.identifier.issn1214-0287en_US
dc.identifier.issue1en_US
dc.identifier.pmid34907704en_US
dc.identifier.scopus2-s2.0-85081201362en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage18en_US
dc.identifier.urihttps://doi.org/10.32725/jab.2020.002
dc.identifier.urihttps://hdl.handle.net/20.500.12713/488
dc.identifier.volume18en_US
dc.identifier.wosWOS:000518448700002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAztopal, Nazlıhanen_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherUniv South Bohemiaen_US
dc.relation.ispartofJournal of Applied Biomedicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast Canceren_US
dc.subjectCytotoxicityen_US
dc.subjectFolk Medicineen_US
dc.subjectGenotoxicityen_US
dc.subjectHuman Lymphocyteen_US
dc.subjectHypericum Olympicumen_US
dc.titleToxicity assessment of Hypericum olympicum subsp. olympicum L. on human lymphocytes and breast cancer cell linesen_US
dc.typeArticleen_US

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