Nanoparticle-Terpene Fusion: A Game-Changer in Combating Primary Amoebic Meningoencephalitis Caused by Naegleria fowleri

Basit Öğe Kaydı
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridShaikh, Mohd. Farooq/0000-0001-9865-6224
dc.authoridRajendran, Kavitha/0009-0008-1689-9387
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidShaikh, Mohd. Farooq/H-6029-2019
dc.contributor.authorRajendran, Kavitha
dc.contributor.authorAhmed, Usman
dc.contributor.authorMeunier, Alexia Chloe
dc.contributor.authorShaikh, Mohd Farooq
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAnwar, Ayaz
dc.date.accessioned2024-05-19T14:50:34Z
dc.date.available2024-05-19T14:50:34Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPathogenic Naegleria fowleri (N. fowleri) are opportunistic free-living amoebae and are the causative agents of a very rare but severe brain infection called primary amoebic meningoencephalitis (PAM). The fatality rate of PAM in reported cases is more than 95%. Most of the drugs used againstN. fowleri infections are repurposed drugs. Therefore, a large number of compounds have been tested againstN. fowleri in vitro, but most of the tested compounds showed high toxicity and an inability to cross the blood-brain barrier. Andrographolide, forskolin, and borneol are important natural compounds that have shown various valuable biological properties. In the present study, the nanoconjugates (AND-AgNPs, BOR-AgNPs, and FOR-AgNPs) of these compounds were synthesized and assessed against both stages (trophozoite and cyst) ofN. fowleri for their antiamoebic and cysticidal potential in vitro. In addition, cytotoxicity and host cell pathogenicity were also evaluated in vitro. FOR-AgNPs were the most potent nanoconjugate and showed potent antiamoebic activity againstN. fowleriwith an IC50 of 26.35 mu M. Nanoconjugates FOR-AgNPs, BOR-AgNPs, and AND-AgNPs also significantly inhibit the viability of N. fowleri cysts. Cytotoxicity assessment showed that these nanoconjugates caused minimum damage to human keratinocyte cells (HaCaT cells) at 100 mu g/mL, while also effectively reducing the cytopathogenicity of N. fowleri trophozoites to the HaCaT cells. The outcomes of our experiments have unveiled substantial potential for AND-AgNPs, BOR-AgNPs, and FOR-AgNPs in the realm of developing innovative alternative therapeutic agents to combat infections caused by N. fowleri. This study represents a significant step forward in the pursuit of advanced strategies for managing such amoebic infections, laying the foundation for the development of novel and more effective therapeutic modalities in the fight against free-living amoebae.en_US
dc.description.sponsorshipSunway University; Sunway University, Malaysiaen_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia.en_US
dc.identifier.doi10.1021/acsomega.3c08844
dc.identifier.endpage11607en_US
dc.identifier.issn2470-1343
dc.identifier.issue10en_US
dc.identifier.pmid38497026en_US
dc.identifier.scopus2-s2.0-85186365079en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage11597en_US
dc.identifier.urihttps://doi.org10.1021/acsomega.3c08844
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5749
dc.identifier.volume9en_US
dc.identifier.wosWOS:001180094400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Omegaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectFree-Living Amebasen_US
dc.subjectPathogenesisen_US
dc.subjectEfficacyen_US
dc.subjectGrowthen_US
dc.subjectDesignen_US
dc.subjectDrugsen_US
dc.titleNanoparticle-Terpene Fusion: A Game-Changer in Combating Primary Amoebic Meningoencephalitis Caused by Naegleria fowlerien_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu