Effect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injury

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorDemirayak, Bengi
dc.contributor.authorYüksel, Nurşen
dc.contributor.authorÇelik, Onur Sinan
dc.contributor.authorSubaşı, Cansu
dc.contributor.authorDuruksu, Gökhan
dc.contributor.authorÜnal, Z. Seda
dc.contributor.authorYıldız, Demir Kürşat
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-08-30T20:08:23Z
dc.date.available2020-08-30T20:08:23Z
dc.date.issued2016
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractIn the present study, we investigate and compare the efficacy of bone marrow- and adipose tissue-derived mesenchymal stem cell (MSCs) in corneal wound healing. A penetrating injury was created in the right corneas of Wistar rats (n = 40). Ten microliters of phosphate-buffered solution (PBS) containing 2 x 10(5) green fluorescent protein (GFP) labeled bone-marrow-derived MSCs to group 1 (n = 15), 10 mu l of PBS containing 2 x 10(5) GFP-labeled adipose-tissue-derived MSCs to group 2 (n = 15), 10 mu l PBS was injected into anterior chamber in group 3 (n = 10, control). Corneal opacity scoring, in vivo confocal microscopy, and histopathological evaluation were done at the end of 8 weeks. Immunofluorescence sections were evaluated to detect transplanted cells. Immune staining was performed to measure the expression levels of keratocan, aldehyde dehydrogenase (ALDH) and CD34. The gene expression levels of tumor necrosis factor (TNF-alpha), the interleukin 6 receptor (IL-6R), interleukin 12b (IL-12b), and transforming growth factor beta (TGF-beta 1) was measured on corneas. The establishment of stem cells in the corneas of the transplanted groups was confirmed by immunofluorescence staining. The expression of keratocan, ALDH, and CD34 increased in the transplanted groups (p < 0.05). The density of keratocytes increased significantly in both transplanted groups according to the in vivo confocal microscopy data (p < 0.05). The expression of TNF-alpha, IL-6R, and IL-12b decreased significantly in the transplanted groups (p < 0.05). Based on our findings, we consider that allogeneic stem cells facilitate the regeneration of corneal stroma and can be a cell source for stromal repopulation in diseased cornea. (C) 2016 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipKocaeli University Scientific Research Projects Unit (KOU-BAP)Kocaeli University [2010-047]en_US
dc.description.sponsorshipThis study was supported by grants from the Kocaeli University Scientific Research Projects Unit (KOU-BAP project no: 2010-047). English editing was supported by Elsevier editing system. We thank to Ozgul Altintas for the statistical analysis.en_US
dc.identifier.citationDemirayak, B., Yüksel, N., Çelik, O. S., Subaşı, C., Duruksu, G., Unal, Z. S., ... & Karaöz, E. (2016). Effect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injury. Experimental eye research, 151, 227-235.en_US
dc.identifier.doi10.1016/j.exer.2016.08.011en_US
dc.identifier.endpage235en_US
dc.identifier.issn0014-4835en_US
dc.identifier.issn1096-0007en_US
dc.identifier.pmid27567556en_US
dc.identifier.scopus2-s2.0-84986587825en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage227en_US
dc.identifier.urihttps://doi.org/10.1016/j.exer.2016.08.011
dc.identifier.urihttps://hdl.handle.net/20.500.12713/929
dc.identifier.volume151en_US
dc.identifier.wosWOS:000385607300028en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofExperimental Eye Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCorneaen_US
dc.subjectKeratocanen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectStromal Wound Healingen_US
dc.titleEffect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injuryen_US
dc.typeArticleen_US

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