Multilayered mesoporous composite nanostructures for highly sensitive label-free quantification of cardiac troponin-I

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dc.authoridAli Zarrabi / 0000-0003-0391-1769en_US
dc.authorscopusidAli Zarrabi / 23483174100
dc.authorwosidAli Zarrabi / U-2602-2019en_US
dc.contributor.authorSaeidi, Mohsen
dc.contributor.authorAmidian, Mohammad Ali
dc.contributor.authorSheybanikashani, Sana
dc.contributor.authorMahdavi, Hossein
dc.contributor.authorAlimohammadi, Homayoon
dc.contributor.authorSyedmoradi, Leila
dc.contributor.authorMohandes, Fatemeh
dc.contributor.authorZarrabi, Ali
dc.contributor.authorTamjid, Elnaz
dc.contributor.authorOmidfar, Kobra
dc.contributor.authorSimchi, Abdolreza
dc.date.accessioned2022-06-02T10:31:37Z
dc.date.available2022-06-02T10:31:37Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.description.abstractCardiac troponin-I (cTnI) is a well-known biomarker for the diagnosis and control of acute myocardial infarction in clinical practice. To improve the accuracy and reliability of cTnI electrochemical immunosensors, we propose a multilayer nanostructure consisting of Fe3O4 -COOH labeled anti-cTnI monoclonal antibody (Fe3O4 -COOH-Ab1 ) and anti-cTnI polyclonal antibody (Ab2 ) conjugated on Au-Ag nanoparticles (NPs) decorated on a metal–organic framework (Au-Ag@ZIF67-Ab2 ). In this design, Fe3O4 -COOH was used for separation of cTnI in specimens and signal amplification, hierarchical porous ZIF-67 extremely enhanced the specific surface area, and Au-Ag NPs synergically promoted the conductivity and sensitivity. They were additionally employed as an immobilization platform to enhance antibody loading. Electron microscopy images indicated that Ag-Au NPs with an average diameter of 1.9 ± 0.5 nm were uniformly decorated on plate-like ZIF-67 particles (with average size of 690 nm) without any agglomeration. Several electrochemical assays were implemented to precisely evaluate the immunosensor performance. The square wave voltammetry technique exhibited the best performance with a sensitivity of 0.98 mA mL cm?2 ng?1 and a detection limit of 0.047 pg mL?1 in the linear range of 0.04 to 8 ng mL?1 .en_US
dc.identifier.citationSaeidi M, Amidian MA, Sheybanikashani S, Mahdavi H, Alimohammadi H, Syedmoradi L, Mohandes F, Zarrabi A, Tamjid E, Omidfar K, Simchi A. Multilayered Mesoporous Composite Nanostructures for Highly Sensitive Label-Free Quantification of Cardiac Troponin-I. Biosensors (Basel). 2022 May 14;12(5):337. doi: 10.3390/bios12050337. PMID: 35624638; PMCID: PMC9138364.en_US
dc.identifier.doi10.3390/bios12050337en_US
dc.identifier.issn2079-6374en_US
dc.identifier.issue5en_US
dc.identifier.pmid9138364en_US
dc.identifier.scopus2-s2.0-85130747145en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttp://doi.org/10.3390/bios12050337.
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2782
dc.identifier.volume12en_US
dc.identifier.wosWOS:000802761000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZarrabi, Ali
dc.language.isoenen_US
dc.publisherPMCen_US
dc.relation.ispartofBiosensorsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectElectrochemical Immunosensorsen_US
dc.subjectCardiac Troponin-Ien_US
dc.subjectHeart Attack Risken_US
dc.subjectGold and Silver Decorated ZIF-67en_US
dc.titleMultilayered mesoporous composite nanostructures for highly sensitive label-free quantification of cardiac troponin-Ien_US
dc.typeArticleen_US

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