Combination of histone deacetylase inhibitor with Cu(II) 5,5- diethylbarbiturate complex induces apoptosis in breast cancer stem cells: a promising novel approach

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authoridNazlıhan Aztopal / 0000-0003-3118-8061
dc.authoridMerve Erkısa Genel / 0000-0002-3127-742X
dc.authorscopusidNazlıhan Aztopal / 55853882900
dc.authorscopusidMerve Erkısa / 57126208900
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidNazlıhan Aztopal / L-6687-2018
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.authorwosidMerve Erkısa / AAM-1001-2020
dc.contributor.authorErkısa Genel, Merve
dc.contributor.authorAztopal, Nazlıhan
dc.contributor.authorErtürk, Elif
dc.contributor.authorUlukaya, Engin
dc.contributor.authorYılmaz, Veysel Turan
dc.contributor.authorAri, Ferda
dc.date.accessioned2020-12-17T07:43:26Z
dc.date.available2020-12-17T07:43:26Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Rektörlüken_US
dc.description.abstractBackground: Cancer stem cells (CSC) are subpopulation within the tumor that acts a part in the initiation, progression, recurrence, resistance to drugs and metastasis of cancer. It is well known that epigenetic changes lead to tumor formation in cancer stem cells and show drug resistance. Epigenetic modulators and /or their combination with different agents have been used in cancer therapy. Objective: In our study we scope out the effects of combination of a histone deacetylases inhibitor, valproic acid (VPA), and Cu(II) complex [Cu(barb-?N)(barb-?2N,O)(phen-?N,N')]·H2O] on cytotoxicity/apoptosis in a stem-cell enriched population (MCF-7s) obtained from parental breast cancer cell line (MCF-7). Methods: Viability of the cells was measured by the ATP assay. Apoptosis was elucidated via the assessment of caspase-cleaved cytokeratin 18 (M30 ELISA) and a group of flow cytometry analysis (caspase 3/7 activity, phosphatidylserine translocation by annexin V-FITC assay, DNA damage and oxidative stress) and 2',7'-dichlorofluorescein diacetate staining. Results: The VPA combined with Cu(II) complex showed anti proliferative activity on MCF-7s cells in a dose- and time-dependently. Treatment with combination of 2.5 mM VPA and 3.12 ?M Cu(II) complex induces oxidative stress in a time-dependent manner, as well as apoptosis that is evidenced by the increase in caspase 3/7 activity, positive annexin-V-FITC, and increase in M30 levels. Conclusion: The results suggest that the combination therapy induces apoptosis following increased oxidative stress, thereby making it a possible promising therapeutic strategy that further analysis is required.en_US
dc.identifier.citationErkisa, M., Aztopal, N., Erturk, E., Ulukaya, E., Yilmaz, V. T., & Ari, F. (2020). Combination of Histone Deacetylase Inhibitor with Cu(II) 5,5- diethylbarbiturate Complex Induces Apoptosis in Breast Cancer Stem Cells: A Promising Novel Approach. Anti-cancer agents in medicinal chemistry, 10.2174/1871520621666201207090702. Advance online publication. https://doi.org/10.2174/1871520621666201207090702en_US
dc.identifier.doi10.2174/1871520621666201207090702en_US
dc.identifier.issn1871-5206en_US
dc.identifier.issn1875-5992en_US
dc.identifier.pmid33292140en_US
dc.identifier.scopus2-s2.0-85111166259en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.2174/1871520621666201207090702
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1279
dc.identifier.wosWOS:000674433600008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErkısa Genel, Merve
dc.institutionauthorAztopal, Nazlıhan
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.relation.ispartofAnti-cancer agents in medicinal chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCu(II) Complexen_US
dc.subjectEpigenetic Modulatorsen_US
dc.subjectApoptosisen_US
dc.subjectBreast Cancer Stem Cellen_US
dc.subjectHistone Deacetylase Inhibitoren_US
dc.subjectValproic Aciden_US
dc.titleCombination of histone deacetylase inhibitor with Cu(II) 5,5- diethylbarbiturate complex induces apoptosis in breast cancer stem cells: a promising novel approachen_US
dc.typeArticleen_US

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