Fluorescence anisotropy cytosensing of folate receptor positive tumor cells using 3D polyurethane-GO-foams modified with folic acid: molecular dynamics and in vitro studies

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dc.authoridAli Zarrabi / 0000-0003-0391-1769en_US
dc.authorscopusidAli Zarrabi / 23483174100en_US
dc.authorwosidAli Zarrabi / U-2602-2019en_US
dc.contributor.authorEsmaeili, Yasaman
dc.contributor.authorMohammadi, Zahra
dc.contributor.authorKhavani, Mohammad
dc.contributor.authorSanati, Alireza
dc.contributor.authorShariati, Laleh
dc.contributor.authorSeyedhosseini Ghaheh, Hooria
dc.contributor.authorBidram, Elham
dc.contributor.authorZarrabi, Ali
dc.date.accessioned2023-02-03T07:53:08Z
dc.date.available2023-02-03T07:53:08Z
dc.date.issued2023en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.description.abstractIntegrated polyurethane (PU)-based foams modified with PEGylated graphene oxide and folic acid (PU@GO-PEG-FA) were developed with the goal of capturing and detecting tumor cells with precision. The detection of the modified PU@GO-PEG surface through FA against folate receptor-overexpressed tumor cells is the basis for tumor cell capture. Molecular dynamics (MD) simulations were applied to study the strength of FA interactions with the folate receptor. Based on the obtained results, the folate receptor has intense interactions with FA, which leads to the reduction in the FA interactions with PEG, and so decreases the fluorescence intensity of the biosensor. The synergistic interactions offer the FA-modified foams a high efficiency for capturing the tumor cell. Using a turn-off fluorescence technique based on the complicated interaction of FA-folate receptor generated by target recognition, the enhanced capture tumor cells could be directly read out at excitation-emission wavelengths of 380-450 nm. The working range is between 1×10 2 to 2×10 4 cells mL -1 with a detection limit of 25 cells mL -1 and good reproducibility with relative standard deviation of 2.35%. Overall, findings demonstrate that the fluorescence-based biosensor has a significant advantage for early tumor cell diagnosis. © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.en_US
dc.identifier.citationEsmaeili, Y., Mohammadi, Z., Khavani, M., Sanati, A., Shariati, L., Seyedhosseini Ghaheh, H., ... & Zarrabi, A. (2023). Fluorescence anisotropy cytosensing of folate receptor positive tumor cells using 3D polyurethane-GO-foams modified with folic acid: molecular dynamics and in vitro studies. Microchimica Acta, 190(1), 44.en_US
dc.identifier.doi10.1007/s00604-022-05558-1en_US
dc.identifier.issn1436-5073en_US
dc.identifier.issue1en_US
dc.identifier.pmid36602637en_US
dc.identifier.scopus2-s2.0-85145668851en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttp://doi.org/10.1007/s00604-022-05558-1
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3868
dc.identifier.volume190en_US
dc.identifier.wosWOS:000909605900003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZarrabi, Ali
dc.language.isoenen_US
dc.publisherNLM (Medline)en_US
dc.relation.ispartofMikrochimica actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytosensoren_US
dc.subjectEarly Tumor Cell Detectionen_US
dc.subjectFluorescence Detectionen_US
dc.subjectMolecular Dynamic Simulationen_US
dc.subjectSwitch On/Offen_US
dc.titleFluorescence anisotropy cytosensing of folate receptor positive tumor cells using 3D polyurethane-GO-foams modified with folic acid: molecular dynamics and in vitro studiesen_US
dc.typeArticleen_US

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