Cell death of Acanthamoeba castellanii following exposure to antimicrobial agents commonly included in contact lens disinfecting solutions

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dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridLloyd, David/0000-0002-5656-0571
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.contributor.authorThomas, Louise
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAlawfi, Bader S.
dc.contributor.authorLloyd, David
dc.date.accessioned2024-05-19T14:38:46Z
dc.date.available2024-05-19T14:38:46Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractSeveral antimicrobial agents are commonly included in contact lens disinfectant solutions including chlorhexidine diacetate (CHX), polyhexamethylene biguanide (PHMB) or myristamidopropyl dimethylamine (MAPD); however, their mode of action, i.e. necrosis versus apoptosis is incompletely understood. Here, we determined whether a mechanism of cell death resembling that of apoptosis was present in Acanthamoeba castellanii of the T4 genotype (NEFF) following exposure to the aforementioned antimicrobials using the anticoagulant annexin V that undergoes rapid high affinity binding to phosphatidylserine in the presence of calcium, making it a sensitive probe for phosphatidylserine exposure. The results revealed that under the conditions employed in this study, an apoptotic pathway of cell death in this organism at the tested conditions does not occur. Our findings suggest that necrosis is the likely mode of action; however, future mechanistic studies should be accomplished in additional experimental conditions to further comprehend the molecular mechanisms of cell death in Acanthamoeba.en_US
dc.description.sponsorshipAir Force Office of Scientific Research (AFOSR), USA [76134-2099]en_US
dc.description.sponsorshipThe authors wish to thank Alcon Research Ltd., Fort Worth, Texas 76134-2099, USA, for providing the MAPD and also Dr. Andrew Hollins, Welsh School of Pharmacy, for his help with the flow cytometry.en_US
dc.identifier.doi10.1007/s00436-023-08061-5
dc.identifier.issn0932-0113
dc.identifier.issn1432-1955
dc.identifier.issue1en_US
dc.identifier.pmid38060008en_US
dc.identifier.scopus2-s2.0-85178951209en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1007/s00436-023-08061-5
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4599
dc.identifier.volume123en_US
dc.identifier.wosWOS:001117590600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofParasitology Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcanthamoeba Castellaniien_US
dc.subjectAmoebicidalen_US
dc.subjectTrophicidalen_US
dc.subjectContact Lens Disinfectantsen_US
dc.subjectKeratitisen_US
dc.titleCell death of Acanthamoeba castellanii following exposure to antimicrobial agents commonly included in contact lens disinfecting solutionsen_US
dc.typeArticleen_US

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