The effect of mesenchymal stem cells administration on DNA repair gene expressions in critically ill COVID-19 patients: prospective controlled study

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dc.contributor.authorIsiksacan, Nilgun
dc.contributor.authorAdas, Gokhan
dc.contributor.authorKasapoglu, Pinar
dc.contributor.authorCukurova, Zafer
dc.contributor.authorYilmaz, Rabia
dc.contributor.authorYasar, Kadriye Kurt
dc.contributor.authorKoyuncu, Duygu Irmak
dc.date.accessioned2024-05-19T14:40:31Z
dc.date.available2024-05-19T14:40:31Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractWhen the studies are evaluated, immunomodulatory effect of MSCs, administration in critically ill patients, obstacle situations in use and side effects, pulmonary fibrosis prevention, which stem cells and their products, regeneration effect, administration route, and dosage are listed under the main heading like. The effect of MSC administration on DNA repair genes in COVID-19 infection is unknown. Our aim is to determine the effect of mesenchymal stem cells (MSCs) therapy applied in critically ill patients with coronavirus infection on DNA repair pathways and genes associated with those pathways. Patients (n = 30) divided into two equal groups. Group-1: Patients in a critically ill condition, Group-2: Patients in critically ill condition and transplanted MSCs. The mechanism was investigated in eleven genes of five different pathways; Base excision repair: PARP1, Nucleotide excision repair (NER): RAD23B and ERCC1, Homologous recombinational repair (HR): ATM, RAD51, RAD52 and WRN, Mismatch repair (MMR): MLH1, MSH2, and MSH6, Direct reversal repair pathway: MGMT. It was found that MSCs application had a significant effect on 6 genes located in 3 different DNA damage response pathways. These are NER pathway genes; RAD23 and ERCC1, HR pathway genes; ATM and RAD51, MMR pathway genes; MSH2 and MSH6 (p < 0.05). Two main points were shown. First, as a result of cellular damage in critical patients with COVID-19, DNA damage occurs and then DNA repair pathways and genes are activated in reaction to this situation. Second, administration of MSC to patients with COVID-19 infection plays a positive role by increasing the expression of DNA repair genes located in DNA damage pathways.en_US
dc.description.sponsorshipHealth Institutes of Turkey in 2020 [8860/9193]en_US
dc.description.sponsorshipThis work was supported by the Health Institutes of Turkey in 2020 (number: 8860/9193).en_US
dc.identifier.doi10.1080/15257770.2024.2327478
dc.identifier.issn1525-7770
dc.identifier.issn1532-2335
dc.identifier.pmid38459810en_US
dc.identifier.scopus2-s2.0-85187115630en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org10.1080/15257770.2024.2327478
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4975
dc.identifier.wosWOS:001181737700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofNucleosides Nucleotides & Nucleic Acidsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectCovid-19en_US
dc.subjectCritical Patienten_US
dc.subjectDna Repair Genesen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.titleThe effect of mesenchymal stem cells administration on DNA repair gene expressions in critically ill COVID-19 patients: prospective controlled studyen_US
dc.typeArticleen_US

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