Comparative proteomics analysis of four commonly used methods for identification of novel plasma membrane proteins

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorYöneten, Kübra Karaosmanoğlu
dc.contributor.authorKasap, Murat
dc.contributor.authorAkpınar, Gürler
dc.contributor.authorKanlı, Aylin
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-08-30T20:06:26Zen_US
dc.date.available2020-08-30T20:06:26Zen_US
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractPlasma membrane proteins perform a variety of important tasks in the cells. These tasks can be diverse as carrying nutrients across the plasma membrane, receiving chemical signals from outside the cell, translating them into intracellular action, and anchoring the cell in a particular location. When these crucial roles of plasma membrane proteins are considered, the need for their characterization becomes inevitable. Certain characteristics of plasma membrane proteins such as hydrophobicity, low solubility, and low abundance limit their detection by proteomic analyses. Here, we presented a comparative proteomics study in which the most commonly used plasma membrane protein enrichment methods were evaluated. The methods that were utilized include biotinylation, selective CyDye labeling, temperature-dependent phase partition, and density-gradient ultracentrifugation. Western blot analysis was performed to assess the level of plasma membrane protein enrichment using plasma membrane and cytoplasmic protein markers. Quantitative evaluation of the level of enrichment was performed by two-dimensional electrophoresis (2-DE) and benzyldimethyl-n-hexadecylammonium chloride/sodium dodecyl sulfate polyacrylamide gel electrophoresis (16-BAC/SDS-PAGE) from which the protein spots were cut and identified. Results from this study demonstrated that density-gradient ultracentrifugation method was superior when coupled with 16-BAC/SDS-PAGE. This work presents a valuable contribution and provides a future direction to the membrane sub-proteome research by evaluating commonly used methods for plasma membrane protein enrichment.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113S868]en_US
dc.identifier.citationYoneten, K. K., Kasap, M., Akpinar, G., Kanli, A., & Karaoz, E. (2019). Comparative Proteomics Analysis of Four Commonly Used Methods for Identification of Novel Plasma Membrane Proteins. The Journal of membrane biology, 252(6), 587-608.en_US
dc.identifier.doi10.1007/s00232-019-00084-3en_US
dc.identifier.endpage608en_US
dc.identifier.issn0022-2631en_US
dc.identifier.issn1432-1424en_US
dc.identifier.issue6en_US
dc.identifier.pmid31346646en_US
dc.identifier.scopus2-s2.0-85069684765en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage587en_US
dc.identifier.urihttps://doi.org/10.1007/s00232-019-00084-3en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/516en_US
dc.identifier.volume252en_US
dc.identifier.wosWOS:000492416500007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Membrane Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlasma Membrane Proteinsen_US
dc.subjectMembrane Protein Enrichmenten_US
dc.subject2-Deen_US
dc.subject16-Bacen_US
dc.subjectSds-Pageen_US
dc.titleComparative proteomics analysis of four commonly used methods for identification of novel plasma membrane proteinsen_US
dc.typeArticleen_US

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