NFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: a case-control study

Basit Öğe Kaydı
dc.authoridFatma Kübra Tombultürk / 0000-0002-4358-2309en_US
dc.authorscopusidFatma Kübra Tombultürk / 56748052500
dc.authorwosidFatma Kübra Tombultürk / L-3969-2018
dc.contributor.advisorÖzkara, Hamdi
dc.contributor.advisorKanıgür-Sultuybek, Gönül
dc.contributor.authorTunçdemir, Matem
dc.contributor.authorYenmiş, Güven
dc.contributor.authorTombultürk, Fatma Kübra
dc.contributor.authorArkan, Hülya
dc.contributor.authorSoydaş, Tuğba
dc.contributor.authorTek, Raşit Burak
dc.contributor.authorAltıntaş, Özlem
dc.date.accessioned2020-08-30T20:07:37Z
dc.date.available2020-08-30T20:07:37Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümüen_US
dc.description.abstractBackground: A notable proportion of idiopathic male infertility cases is accompanied by oligozoospermia; and yet, the molecular mechanisms of fertilization problem underlying this defect are still unclear. Epithelial cadherin has been involved in several calcium-dependent cell-to-cell adhesion events; however, its participation in gamete interaction has also not been fully investigated. Objective: The aim was to investigate the changes in the expression of E-cadherin, based on the frequency of Single nucleotide polymorphisms in Nuclear Factor Kappa-B 1 and pre-mir-146a in oligospermic men. Materials and Methods: In this case-control study, semen and blood samples of 131 oligospermic men as the case group and 239 fertile healthy men as the control group were analyzed. Variants single nucleotide polymorphisms rs28362491 and rs2910164 were performed using polymerase chain reaction-restriction fragment length polymorphism method and E-cadherin expression were determined by immunoprecipitation studies. Results: ins/ins genotype of rs28362491 was determined as a risk factor for idiopathic oligospermia by 1.73 times (p=0.0218), whereas no significant differences were found between the groups concerning pre-mir-146a rs2910164 polymorphism (p=0.2274 in case of GC genotype and p=0.9052 in case of GG genotype). Combined genotype analysis results did not show any notable differences between the multiple comparisons of 28362491-rs2910164 in oligospermic men and control groups. In addition, E-cadherin expression of oligospermic men with ins/ins genotype was significantly lower than patients with del/ins genotype (p=0.0221). E-cadherin expression level was low in oligospermic men with respect to the control group in presence of ins/ins genotype of NFKB1 gene. Conclusion: These results suggest that ins allele prevents binding of surface proteins to spermatozoa, leading to a low affinity of sperm-oocyte interaction in oligospermic men.en_US
dc.description.sponsorshipIstanbul University Research FundIstanbul University [24764]en_US
dc.description.sponsorshipThis study is supported by Istanbul University Research Fund (project number 24764). Tuncdemir, Tombulturk, and Altintas did the immunocytochemistry part of the study. Yenmis and Soydas did the PCR and the RFLP of the study. Yenmis and Tuncdemir also had the major role in writing with Kanigur. Arkan did the statistics and helped RFLP part. Tek and Ozkara as clinicians diagnosed the oligospermic patients. Kanigur was responsible for the supervising the whole study.en_US
dc.identifier.citationTunçdemir, M., Yenmiş, G., Tombultürk, K., Arkan, H., Soydaş, T., Tek, R. B., ... & Kanıgür-Sultuybek, G. (2018). NFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: A case-control study. International Journal of Reproductive BioMedicine, 16(4), 247.en_US
dc.identifier.doi10.29252/ijrm.16.4.247en_US
dc.identifier.endpage254en_US
dc.identifier.issn2476-4108en_US
dc.identifier.issn2476-3772en_US
dc.identifier.issue4en_US
dc.identifier.pmid29942932en_US
dc.identifier.scopus2-s2.0-85047902614en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage247en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/801
dc.identifier.urihttps://doi.org/10.29252/ijrm.16.4.247en_US
dc.identifier.volume16en_US
dc.identifier.wosWOS:000433283100003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorTombultürk, Fatma Kübraen_US
dc.language.isoenen_US
dc.publisherShahid Sadoughi Univ Medical Sciencesen_US
dc.relation.ispartofInternational Journal of Reproductive Biomedicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectE-Cadherinen_US
dc.subjectOligospermiaen_US
dc.subjectMale Infertilityen_US
dc.subjectNuclear Factor Kappa Ben_US
dc.subjectMir-146aen_US
dc.titleNFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: a case-control studyen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
H129.pdf
Boyut:
641.78 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıbbi Laboratuvar Teknikleri Programı Makale Koleksiyonu