Time kill-assays of antibiotic combinations for multidrug resistant clinical isolates of OXA-48 carbapenemase producing Klebsiella pneumoniae

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dc.authoridAyham Abulaila / 0000-0001-9615-3391en_US
dc.authorscopusidAyham Abulaila / 57191905263
dc.authorwosidAyham Abulaila / AAV-4591-2020en_US
dc.contributor.authorErdem, Fatma
dc.contributor.authorDíez-Aguilar, María
dc.contributor.authorÖksüz, Lütfiye
dc.contributor.authorKayacan,Çiğdem
dc.contributor.authorAbulaila, Ayham
dc.contributor.authorÖncül, Oral
dc.contributor.authorMorosini, María Isabel
dc.contributor.authorCantón, Rafael
dc.contributor.authorAktaş, Zerrin
dc.date.accessioned2022-07-30T11:13:51Z
dc.date.available2022-07-30T11:13:51Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractTreatment of infections caused by OXA-48 carbapenemase producing multidrug-resistant isolates often necessitates combination therapy. In vitro effect of different antibiotic combinations against multidrug-resistant (MDR) Klebsiella pneumoniae isolates were evaluated in this study. Meropenem-tobramycin (MER+TOB), meropenem-ciprofloxacin (MER+CIP), colistin-meropenem (COL+MER), colistin-ciprofloxacin (COL+CIP) and colistin-tobramycin (COL+TOB) combinations were tested by time kill-assays. Each antibiotic alone and in combination at their Cmax values were tested against 4 clinical K. pneumoniae isolates at 1, 2, 4, 6, 8, 12 and 24 h. Effect of colistin and its associations were also assessed at 30 min. Bactericidal activity was defined as ?3log10 CFU mL-1 decrease compared with initial inoculum. Synergy was defined as ?2log10CFU mL-1 decrease by the combination compared with the most active single agent. Presence of bla OXA-48, bla NDM, bla VIM, bla IMP, bla KPC and bla CTX-M-1 genes was screened by PCR using specific primers. The bla OXA-48 gene was identified together with bla CTXM-1 group gene in all isolates. COL+MER demonstrated to be synergistic and bactericidal. MER+TOB showed synergistic and bactericidal effect on two strains although, regrowth was seen on other two strains at 24 h. MER+CIP exhibited indifferent effect on the strains. Combination therapy could be a potential alternative to treat MDR K. pneumoniae infections. This combination might prevent resistance development and secondary effects of colistin monotherapy. MER+TOB and MER+CIP might have an isolate-dependent effect, that may not always result in synergismen_US
dc.identifier.citationErdem F, Díez-Aguilar M, Oksuz L, Kayacan C, Abulaila A, Oncul O, Morosini MI, Cantón R, Aktas Z. Time kill-assays of antibiotic combinations for multidrug resistant clinical isolates of OXA-48 carbapenemase producing Klebsiella pneumoniae. Acta Microbiol Immunol Hung. 2022 Jun 14. doi: 10.1556/030.2022.01785. Epub ahead of print. PMID: 35895557.en_US
dc.identifier.doi10.1556/030.2022.01785en_US
dc.identifier.issn1217-8950en_US
dc.identifier.pmid35895557en_US
dc.identifier.scopus2-s2.0-85139864940en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttp://doi.org/10.1556/030.2022.01785
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3060
dc.identifier.wosWOS:000869448500006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAbulaila, Ayham
dc.language.isoenen_US
dc.publisherAkJournalsen_US
dc.relation.ispartofActa Microbiologica et Immunologica Hungaricaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMDR Klebsiella Pneumoniaeen_US
dc.subjectOXA-48 Carbapenemaseen_US
dc.subjectCombination Therapyen_US
dc.subjectTime Kill-Assayen_US
dc.titleTime kill-assays of antibiotic combinations for multidrug resistant clinical isolates of OXA-48 carbapenemase producing Klebsiella pneumoniaeen_US
dc.typeArticleen_US

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