Effect of vitamin B12 on methotrexate-induced cardiotoxicity in rats

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dc.authoridCEYLAN, TAYFUN/0000-0002-0917-0378
dc.authorwosidCEYLAN, TAYFUN/AAF-1001-2021
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorKarabulut, Derya
dc.contributor.authorKaymak, Emin
dc.contributor.authorAkin, Ali Tugrul
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorYildirim, Aysegul Burcin
dc.contributor.authorYakan, Birkan
dc.date.accessioned2024-05-19T14:41:21Z
dc.date.available2024-05-19T14:41:21Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractObjective(s): Methotrexate (MTX) is a drug with anti-inflammatory and immunosuppressive effects and is also a folic acid antagonist. Our aim in this study is to determine the molecular mechanisms of cardiotoxicity caused by MTX, a chemotherapeutic drug, and to evaluate the protective effects of vitamin B12 on this toxicity. Materials and Methods: A total of 32 rats were used in our study and 4 groups were formed. Control group, Vit B12 group (3 mu g/kg B12 for 15 days, IP), MTX group (20 mg/kg MTX single dose on day 8 of the experiment, IP), MTX +Vit B12 group (3 mu g/kg, IP ), Vit B12 throughout the 15 days, and a single dose of 20 mg/kg MTX (IP) on day 8 of the experiment. Immunohistochemically, expressions of hypoxia-inducible factor 1 alpha (HIF1-alpha), vascular endothelial growth factor receptor -2 (VEGFR-2), erythropoietin (EPO), and interleukin-6 (IL -6) were evaluated in the heart tissue. Total catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) levels were measured in the heart tissue. At the same time, ANP and NT-proBNP levels were measured in the blood serum. Results: In the study, the expression of HIF1-alpha and VEGFR-2 increased significantly in the MTX group, while IL -6 and EPO significantly decreased. At the same time, CAT and SOD levels were significantly decreased and MDA levels increased significantly in the MTX group. While vitamin B12 significantly corrected all these values, it also greatly reduced the increases in ANP and NT-proBNP levels caused by MTX. Conclusion: It is important to use Vit B12 before and after MTX administration to replace the folate that MTX has reduced.en_US
dc.description.sponsorshipScientific Research Projects Unit of Erciyes University, Turkiye [TSA-2019-8673]en_US
dc.description.sponsorshipThis work was financially supported by the Scientific Research Projects Unit of Erciyes University, Turkiye (TSA-2019-8673 project code).en_US
dc.identifier.doi10.22038/IJBMS.2024.74161.16120
dc.identifier.endpage739en_US
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue6en_US
dc.identifier.pmid38645491en_US
dc.identifier.scopus2-s2.0-85188176204en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage733en_US
dc.identifier.urihttps://doi.org10.22038/IJBMS.2024.74161.16120
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5098
dc.identifier.volume27en_US
dc.identifier.wosWOS:001187385400014en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMashhad Univ Med Sciencesen_US
dc.relation.ispartofIranian Journal of Basic Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectErythropoietin Hypoxiaen_US
dc.subjectInducible Factoren_US
dc.subject1 Methotrexate Vascular Endothelial Growthen_US
dc.subjectFactor Vitamin B12en_US
dc.titleEffect of vitamin B12 on methotrexate-induced cardiotoxicity in ratsen_US
dc.typeArticleen_US

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