Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi

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dc.authoridÖzlem Büyüktanır Yaş / 0000-0002-7641-7350en_US
dc.authorscopusidÖzlem Büyüktanır Yaş / 57221162761
dc.authorwosidÖzlem Büyüktanır Yaş / AAP-2720-2020
dc.contributor.authorKo?i, Juraj
dc.contributor.authorBista, Sandhya
dc.contributor.authorChirania, Payal
dc.contributor.authorYang, Xiuli
dc.contributor.authorKitsou, Chrysoula
dc.contributor.authorBüyüktanır Yaş, Özlem
dc.date.accessioned2021-03-22T12:13:14Z
dc.date.available2021-03-22T12:13:14Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractIxodes scapularis ticks transmit multiple pathogens, including Borrelia burgdorferi sensu stricto, and encode many proteins harboring epidermal growth factor (EGF)-like domains. We show that I. scapularis produces multiple orthologs for Bm86, a widely studied tick gut protein considered as a target of an anti-tick vaccine, herein termed as Is86. We show that Is86 antigens feature at least three identifiable regions harboring EGF-like domains (termed as EGF-1, EGF-2, and EGF-3) and are differentially upregulated during B. burgdorferi infection. Although the RNA interference-mediated knockdown of Is86 genes did not show any influences on tick engorgement or B. burgdorferi sensu stricto persistence, the immunization of murine hosts with specific recombinant EGF antigens marginally reduced spirochete loads in the skin, in addition to affecting tick blood meal engorgement and molting. However, given the borderline impact of EGF immunization on tick engorgement and pathogen survival in the vector, it is unlikely that these antigens, at least in their current forms, could be developed as potential vaccines. Further investigations of the biological significance of Is86 (and other tick antigens) would enrich our knowledge of the intricate biology of ticks, including their interactions with resident pathogens, and contribute to the development of anti-tick measures to combat tick-borne illnesses.en_US
dc.identifier.citationKoči, J., Bista, S., Chirania, P., Yang, X., Kitsou, C., Rana, V. S., Yas, O. B., Sonenshine, D. E., & Pal, U. (2021). Antibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferi. Scientific reports, 11(1), 6095. https://doi.org/10.1038/s41598-021-85624-5en_US
dc.identifier.doi10.1038/s41598-021-85624-5en_US
dc.identifier.endpage13en_US
dc.identifier.issue6095en_US
dc.identifier.pmid33731754en_US
dc.identifier.scopus2-s2.0-85102765274en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-021-85624-5
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1627
dc.identifier.volume11en_US
dc.identifier.wosWOS:000630509800010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBüyüktanır Yaş, Özlem
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofSci Rep .en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAntibodies against EGF-like domains in Ixodes scapularis BM86 orthologs impact tick feeding and survival of Borrelia burgdorferien_US
dc.typeArticleen_US

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