Preparation and characterization of palladium derivate-loaded micelle formulation in vitro as an innovative therapy option against non-small cell lung cancer cells

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dc.authoridMerve Erkısa Genel / 0000-0002-3127-742X
dc.authoridEngin Ulukaya / 0000-0003-4875-5472
dc.authorscopusidMerve Erkısa Genel / 57126208900
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidMerve Erkısa Genel / AAM-1001-2020
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorErkısa Genel, Merve
dc.contributor.authorArı, Ferda
dc.contributor.authorBüyükköroğlu, Gülay
dc.contributor.authorSenel, Bedriye
dc.contributor.authorYılmaz, Veysel Turan
dc.contributor.authorUlukaya, Engin
dc.date.accessioned2021-08-19T06:40:16Z
dc.date.available2021-08-19T06:40:16Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesien_US
dc.description.abstractNanoparticles have been used in cancer treatments to target tumor and reduce side effects. In this study, we aimed to increase the effectiveness of palladium(II) complex [PdCl(terpy)](sac)·2H2O, which previously showed anticancer potential, by preparing the nanoparticle formulation. An inhalable micellar dispersion containing a palladium(II) complex (PdNP) was prepared and its physicochemical characteristics were evaluated using in vitro tests. Morphology, size and surface charges of particle and loading/encapsulation efficiency of PdNP were analyzed by scanning electron microscopy, zeta sizer and inductively coupled plasma mass spectrometry while aerosol properties of PdNP were measured by the next generation impactor. A549 and H1299 non-small lung cancer cell types were used for cytotoxicity using SRB and ATP assays. Fluorescent staining and M30 antigen assay were carried out for cell death evaluation. Apoptosis was confirmed by flow cytometry analyses. SEM, particle size, and zeta potential results showed the particles have inhalable properties. The amount of the palladium(II) complex loaded into the particles was quantified which indicated high encapsulation efficiencies (97%). The micellar dispersion expected to reach the alveolar region and the brachial region was determined 35% and 47%, respectively. PdNP showed an anti-growth effect by increasing reactive oxygen species that is followed by the induction of mitochondria-dependent apoptosis that is evidenced by pyknotic nuclei and M30 antigen level increments and disruption of polarization of membrane in mitochondria (??m). The results show that PdNP might be a promising inhalable novel complex to be used in non-small cell lung cancer, which warrants animal studies in further.en_US
dc.identifier.citationErkisa, M., Arı, F., Büyükköroğlu, G., Şenel, B., Yılmaz, V. T., & Ulukaya, E. (2021). Preparation and characterization of palladium derivate-loaded micelle formulation in vitro as an innovative therapy option against non-small cell lung cancer cells. Chemistry & biodiversity, 10.1002/cbdv.202100402. Advance online publication. https://doi.org/10.1002/cbdv.202100402en_US
dc.identifier.doi10.1002/cbdv.202100402en_US
dc.identifier.endpage18en_US
dc.identifier.pmid34370383en_US
dc.identifier.scopus2-s2.0-85113337342en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1002/cbdv.202100402
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2006
dc.identifier.wosWOS:000688195600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErkısa Genel, Merve
dc.institutionauthorUlukaya, Engin
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofChemistry & Biodiversityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectLung Canceren_US
dc.subjectNanoparticlesen_US
dc.subjectPalladiumen_US
dc.titlePreparation and characterization of palladium derivate-loaded micelle formulation in vitro as an innovative therapy option against non-small cell lung cancer cellsen_US
dc.typeArticleen_US

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