Gut microbiome of Crocodylus porosus and cellular stress: inhibition of nitric oxide, interleukin 1-beta, tumor necrosis factor-alpha, and prostaglandin E2 in cerebrovascular endothelial cells

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dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridAkbar, Noor/0000-0002-8114-1969
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAkbar, Noor
dc.contributor.authorMaciver, Sutherland K.
dc.contributor.authorAlharbi, Ahmad M.
dc.contributor.authorAlfahemi, Hasan
dc.contributor.authorKhan, Naveed Ahmed
dc.date.accessioned2024-05-19T14:42:03Z
dc.date.available2024-05-19T14:42:03Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractCrocodiles are renowned for their resilience and capacity to withstand environmental stressors, likely influenced by their unique gut microbiome. In this study, we determined whether selected gut bacteria of Crocodylus porosus exhibit anti-inflammatory effects in response to stress, by measuring nitric oxide release, interleukin 1-beta, tumor necrosis factor-alpha, and prostaglandin E2 in cerebrovascular endothelial cells. Using the Griess assay, the findings revealed that among several C. porosus gut bacterial isolates, the conditioned media containing the metabolites of two bacterial strains (CP27 and CP36) inhibited nitric oxide production significantly, in response to the positive control, i.e., taxol-treatment. Notably, CP27 and CP36 were more potent at reducing nitric oxide production than senloytic compounds (fisetin, quercetin). Using enzyme linked immunosorbent assays, the production of pro-inflammatory cytokines (IL-1 beta, TNF-alpha, PGE2), was markedly reduced by treatment with CP27 and CP36, in response to stress. Both CP27 and CP36 contain a plethora of metabolites to exact their effects [(3,4-dihydroxyphenylglycol, 5-methoxytryptophan, nifedipine, 4-chlorotestosterone-17-acetate, 3-phenoxypropionic acid, lactic acid, f-Honaucin A, l,l-Cyclo(leucylprolyl), 3-hydroxy-decanoic acid etc.], indicative of their potential in providing protection against cellular stress. Further high-throughput bioassay-guided testing of gut microbial metabolites from crocodiles, individually as well as in combination, together with the underlying molecular mechanisms, in vitro and in vivo will elucidate their value in the rational development of innovative therapies against cellular stress/gut dysbiosis.en_US
dc.description.sponsorshipAir Force Office of Scientific Research (AFOSR) [FA 8655-20-1-7004]en_US
dc.description.sponsorshipThis work was funded by the Air Force Office of Scientific Research (AFOSR), grant number: FA 8655-20-1-7004.en_US
dc.identifier.doi10.1007/s00203-023-03680-Z
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue10en_US
dc.identifier.pmid37768360en_US
dc.identifier.scopus2-s2.0-85172805611en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1007/s00203-023-03680-Z
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5194
dc.identifier.volume205en_US
dc.identifier.wosWOS:001115402400002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectGut Microbiomeen_US
dc.subjectCrocodileen_US
dc.subjectStressen_US
dc.subjectNitric Oxideen_US
dc.subjectTnf-Alphaen_US
dc.subjectIl-1betaen_US
dc.subjectSenolytic Drugsen_US
dc.titleGut microbiome of Crocodylus porosus and cellular stress: inhibition of nitric oxide, interleukin 1-beta, tumor necrosis factor-alpha, and prostaglandin E2 in cerebrovascular endothelial cellsen_US
dc.typeArticleen_US

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