New approach to microscopy of gram-stained urethral smear: the kissing slide method

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dc.authoridMehmet Sarıer / 0000-0002-8656-7416
dc.authorscopusidMehmet Sarıer / 57193698943
dc.authorwosidMehmet Sarıer / B-8589-2018
dc.contributor.authorSarıer, Mehmet
dc.contributor.authorDemir, Meltem
dc.contributor.authorTurgut, Hasan
dc.contributor.authorHizel, Aydan
dc.contributor.authorEmek, Mestan
dc.contributor.authorKukul, Erdal
dc.contributor.authorSepin, Nevgün
dc.date.accessioned2020-09-24T06:45:42Z
dc.date.available2020-09-24T06:45:42Z
dc.date.issued2020en_US
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Diyaliz Bölümüen_US
dc.description.abstractBackground: The effectiveness of microscopy of Gram-stained smear (GSS) for the detection of male urethral infection is debatable, especially in cases with low inflammation and no visible urethral discharge. This clinical study compared GSS samples collected with the conventional swab method and our new technique, the kissing slide method, together with polymerase chain reaction results to demonstrate the effectiveness of this new method in men with acute urethritis. Methods: The study included 64 men who presented to the urology outpatient clinic with complaints of acute urethritis between October 2019 and January 2020. Two GSS samples were collected from each patient, first using the kissing slide method (applying the slide directly to the urethral mucosa), followed by the conventional method. The results were compared with polymerase chain reaction findings. Results: The patients' mean age was 37.4 ± 7.8 years, and 68.7% had no visible urethral discharge on physical examination. At a GSS threshold of ?5 polymorphonuclear leukocytes/high-power field, sensitivity values were 60% (95% confidence interval [CI], 42.32%-75.41%) for the kissing slide method and 23.33% (95% CI, 11.79%-40.93%) for the conventional method. At a threshold of ?2 polymorphonuclear leukocytes/high-power field, sensitivity values with the kissing slide and conventional methods were 80% (95% CI, 62.69%-90.5%) and 50% (95% CI, 33.15%-66.85%) in all patients, and 66.67% (95% CI, 41.71%-84.82%) and 20% (95% CI, 7.047%-45.19%) in cases without visible urethral discharge, respectively. Conclusion: The new kissing slide method is a noninvasive alternative method that may have better sensitivity than the conventional GSS sampling method in the diagnosis of male acute urethritis. Randomized studies are needed to verify these findings.en_US
dc.identifier.citationSarier, M., Demir, M., Turgut, H., Hizel, A., Emek, M., Kukul, E., & Sepin, N. (2020). New approach to microscopy of Gram-stained urethral smear: the kissing slide method. Sexually Transmitted Diseases, 47(10), 712-715.en_US
dc.identifier.doi10.1097/OLQ.0000000000001228en_US
dc.identifier.endpage715en_US
dc.identifier.issue10en_US
dc.identifier.pmid32649578en_US
dc.identifier.scopus2-s2.0-85091126657en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage712en_US
dc.identifier.urihttps://doi.org/10.1097/OLQ.0000000000001228
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1076
dc.identifier.volume47en_US
dc.identifier.wosWOS:000574642100014en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSarıer, Mehmet
dc.language.isoenen_US
dc.relation.ispartofSex Transm Disen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleNew approach to microscopy of gram-stained urethral smear: the kissing slide methoden_US
dc.typeArticleen_US

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