The effect of recombinant tyrosine hydroxylase expression on the neurogenic differentiation potency of mesenchymal stem cells

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorTuraç, Gizem
dc.contributor.authorDuruksu, Gökhan
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-08-30T20:07:40Z
dc.date.available2020-08-30T20:07:40Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractObjective: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in dopamine synthesis, making the enhancement of its activity a target for ensuring sufficient dopamine levels. Rat bone marrow mesenchymal stem cells (rBM-MSCs) are known to synthesize TH after differentiating into neuronal cells through chemical induction, but the effect of its ectopic expression on these cells has not yet been determined. This study investigated the effects of ectopic recombinant TH expression on the stemness characteristics of rBM-MSCs. Methods: After cloning, a cell line with stable TH expression was maintained, and the proliferation, the gene expression profile, and differentiation potential of rBM-MSCs were analyzed. Analysis of the cells showed an increment in the proliferation rate that could be reversed by the neutralization of TH. Results: The constitutive expression of TH in rBM-MSCs was successfully implemented, without significantly affecting their osteogenic and adipogenic differentiation potential. TH expression improved the expression of other neuronal markers, such as glial fibrillary acidic protein, beta-tubulin, nestin, and c-Fos, confirming the neurogenic differentiation capacity of the stem cells. The expression of brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) significantly increased after the chemical induction of neurogenic differentiation. Conclusion: In this study, the expression of recombinant TH improved the neuroprotective effect of MSCs by upregulating the expression of BDNF and CNTF. Although the neuronal markers were upregulated, the expression of recombinant TH alone in rBM-MSCs was not sufficient for MSCs to differentiate into neurogenic cell lines.en_US
dc.description.sponsorshipScientific and Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111M443]en_US
dc.description.sponsorshipThis study was supported by a grant of the Scientific and Research Council of Turkey (TUBITAK, Grant No. 111M443). We thank Gulay Erman for her help in flow cytometry analysis and Ozlem Saglam for her technical assistance in Western Blot analysis.en_US
dc.identifier.citationTurac, G., Duruksu, G., & Karaoz, E. (2018). The effect of recombinant tyrosine hydroxylase expression on the neurogenic differentiation potency of mesenchymal stem cells. Neurospine, 15(1), 42.en_US
dc.identifier.doi10.14245/ns.1836010.005en_US
dc.identifier.endpage53en_US
dc.identifier.issn2586-6583en_US
dc.identifier.issn2586-6591en_US
dc.identifier.issue1en_US
dc.identifier.pmid29656620en_US
dc.identifier.scopus2-s2.0-85066246915en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage42en_US
dc.identifier.urihttps://doi.org/10.14245/ns.1836010.005
dc.identifier.urihttps://hdl.handle.net/20.500.12713/808
dc.identifier.volume15en_US
dc.identifier.wosWOS:000455717800010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherKorean Spinal Neurosurgery Socen_US
dc.relation.ispartofNeurospineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTyrosine Hydroxylaseen_US
dc.subjectDifferentiationen_US
dc.subjectGene Expressionen_US
dc.subjectNeuroprotectionen_US
dc.subjectMesenchymal Stem Cellen_US
dc.titleThe effect of recombinant tyrosine hydroxylase expression on the neurogenic differentiation potency of mesenchymal stem cellsen_US
dc.typeArticleen_US

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