Repurposing Fc gamma receptor I (Fc?RI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -?)?

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dc.authoridYuce, Meral/0000-0003-0393-1225
dc.authoridYENENLER-KUTLU, ASLI/0000-0002-9169-388X
dc.authorwosidYuce, Meral/J-2892-2019
dc.contributor.authorCapkin, Eda
dc.contributor.authorKutlu, Asli
dc.contributor.authorYuce, Meral
dc.date.accessioned2024-05-19T14:41:28Z
dc.date.available2024-05-19T14:41:28Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe controlled orientation of biomolecules on the sensor surface is crucial for achieving high sensitivity and accurate detection of target molecules in biosensing. Fc gamma RI is an immune cell surface receptor for recognizing IgG-coated targets, such as opsonized pathogens or immune complexes. It plays a crucial role in T cell activation and internalization of the cargos, leading downstream signaling cascades. In this study, we repurposed the Fc gamma RI as an analytical ligand molecule for site-oriented ADA capture, a monoclonal antibody-based biosimilar drug, on a plasmonic sensor surface and demonstrated the real-time detection of the corresponding analyte molecule, TNF-alpha. The study encompasses the analysis of comparative ligand behaviors on the surface, biosensor kinetics, concentration-dependent studies, and sensor specificity assays. The findings of this study suggest that Fc gamma RI has a significant potential to serve as a universal ligand molecule for site-specific monoclonal antibody capture, and it can be used for biosensing studies, as it represents low nanomolar range affinity and excellent selectivity towards the target. However, there is still room for improvement in the surface stability and sensing response, and further studies are needed to reveal its performance on the monoclonal antibodies with various antigen binding sites and glycoforms.en_US
dc.description.sponsorshipTUBITAK 2244 Industrial Ph.D. Program [118C149]en_US
dc.description.sponsorshipE.C. acknowledges TUBITAK 2244 Industrial Ph.D. Program (Grant ID: 118C149) for her scholarship. The authors thank Assoc. Prof. Dr. Hasan Kurt (Senior Research Associate at Imperial College London) and Begum Balkan Apaydin (Personnel at Sabanci University) for useful discussions and technical assistance, respectively.en_US
dc.identifier.doi10.1016/j.heliyon.2023.e19469
dc.identifier.issn2405-8440
dc.identifier.issue9en_US
dc.identifier.pmid37809995en_US
dc.identifier.scopus2-s2.0-85169822507en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org10.1016/j.heliyon.2023.e19469
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5113
dc.identifier.volume9en_US
dc.identifier.wosWOS:001079024100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCell Pressen_US
dc.relation.ispartofHeliyonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectFc Gamma Receptor Ien_US
dc.subjectMonoclonal Antibodyen_US
dc.subjectTnfen_US
dc.subjectAlpha Biosensoren_US
dc.subjectSurface Plasmon Resonanceen_US
dc.titleRepurposing Fc gamma receptor I (Fc?RI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -?)?en_US
dc.typeArticleen_US

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