Anti-Balamuthia mandrillaris and anti-Naegleria fowleri effects of drugs conjugated with various nanostructures

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dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridAlqassim, Saif/0000-0002-1726-1587
dc.authoridJabri, tooba/0000-0001-5889-0913
dc.authorwosidShah, Muhammad Raza/AAR-3981-2021
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorBoghossian, Anania
dc.contributor.authorAlqassim, Saif S.
dc.contributor.authorKawish, Muhammad
dc.contributor.authorGul, Jasra
dc.contributor.authorJabri, Tooba
dc.contributor.authorShah, Muhammad Raza
dc.date.accessioned2024-05-19T14:45:56Z
dc.date.available2024-05-19T14:45:56Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBalamuthia mandrillaris and Naegleria fowleri are protist pathogens that can cause fatal infections. Despite mortality rate of > 90%, there is no effective therapy. Treatment remains problematic involving repurposed drugs, e.g., azoles, amphotericin B and miltefosine but requires early diagnosis. In addition to drug discovery, modifying existing drugs using nanotechnology offers promise in the development of therapeutic interventions against these parasitic infections. Herein, various drugs conjugated with nanoparticles were developed and evaluated for their antiprotozoal activities. Characterizations of the drugs' formulations were accomplished utilizing Fourier-transform infrared spectroscopy, efficiency of drug entrapment, polydispersity index, zeta potential, size, and surface morphology. The nanoconjugates were tested against human cells to determine their toxicity in vitro. The majority of drug nanoconjugates exhibited amoebicidal effects against B. mandrillaris and N. fowleri. Amphotericin B-, Sulfamethoxazole-, Metronidazole-based nanoconjugates are of interest since they exhibited significant amoebicidal effects against both parasites (p < 0.05). Furthermore, Sulfamethoxazole and Naproxen significantly diminished host cell death caused by B. mandrillaris by up to 70% (p < 0.05), while Amphotericin B-, Sulfamethoxazole-, Metronidazole-based drug nanoconjugates showed the highest reduction in host cell death caused by N. fowleri by up to 80%. When tested alone, all of the drug nanoconjugates tested in this study showed limited toxic effects against human cells in vitro (less than 20%). Although these are promising findings, prospective work is warranted to comprehend the mechanistic details of nanoconjugates versus amoebae as well as their in vivo testing, to develop antimicrobials against the devastating infections caused by these parasites.en_US
dc.description.sponsorshipUniversity Internal Grant [CSRG 2021/03]en_US
dc.description.sponsorshipThis work was funded by University Internal Grant: CSRG 2021/03.en_US
dc.identifier.doi10.1007/s00203-023-03518-8
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue5en_US
dc.identifier.pmid37017767en_US
dc.identifier.scopus2-s2.0-85151780362en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1007/s00203-023-03518-8
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5396
dc.identifier.volume205en_US
dc.identifier.wosWOS:000964687600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectNaegleria Fowlerien_US
dc.subjectBalamuthia Mandrillarisen_US
dc.subjectNanocarrieren_US
dc.subjectBlood-Brain Barrieren_US
dc.subjectAnti-Amoebicen_US
dc.subjectMortalityen_US
dc.subjectToxicityen_US
dc.subjectDrug Deliveryen_US
dc.titleAnti-Balamuthia mandrillaris and anti-Naegleria fowleri effects of drugs conjugated with various nanostructuresen_US
dc.typeArticleen_US

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