Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage

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dc.authoridEngin Ulukaya / 0000-0003-4875-5472en_US
dc.authorscopusidEngin Ulukaya / 6602927353
dc.authorwosidEngin Ulukaya / K-5792-2018
dc.contributor.authorAybastıer, Önder
dc.contributor.authorDawbaa, Sam
dc.contributor.authorDemir, Cevdet
dc.contributor.authorAkgün, Oğuzhan
dc.contributor.authorUlukaya, Engin
dc.contributor.authorArı, Ferda
dc.date.accessioned2020-08-30T20:07:41Z
dc.date.available2020-08-30T20:07:41Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractLung cancer has a high treatment cost and poor prognosis in comparison to other types of cancers. This work was involved in studying oxidative DNA base damage inhibition. Accordingly, standard carvacrol, thymol, thymoquinone with water and water-methanol extract of thyme (Origanum vulgare L. subsp. hirtum (link.) Ietswaart), thyme oil and thyme water were prepared and investigated for their efficacy to inhibit DNA oxidative damage formed by H2O2 in malignant lung cells (A549). The antioxidant capacity by ABTS assay was 271.73 +/- 11.45 mg trolox equivalent/mL for thyme oil. HPLC analysis was carried out to determine the contents of different thyme extracts, results showing the presence of carvacrol, thymol, protocatechuic acid, caffeic acid, epicatechin and rosmarinic acid in water and water-methanol extracts while only carvacrol and thymol were found in thyme oil and thyme water. After DNA isolation from the cultured cells, the formed oxidative induced DNA damage products were analysed using GC-MS/MS. It was proven that the antioxidants in the cell culture media have succeeded to inhibit oxidative DNA base damage. Thymoquinone was shown to be the best protectant antioxidant among other antioxidants against the formation of oxidative DNA damage, whereas water-methanol extract of thyme was the best among the plant-sourced samples. Thymoquinone and thyme water-methanol extract were investigated for their efficacy on cultured healthy lung cells (BEAS-2B), and it was proven that they are efficient in protection against the oxidation of DNA of healthy lung cells too.en_US
dc.description.sponsorshipUludag University Research FoundationUludag University [2014/5]en_US
dc.description.sponsorshipThis work was supported by the Uludag University Research Foundation (Project No. 2014/5). We appreciate Prof. Dr. Hakan AKCA (Medical School of Pamukkale University) for supporting us with the cell lines.en_US
dc.identifier.citationAybastier, O., Dawbaa, S., Demir, C., Akgun, O., Ulukaya, E., & Ari, F. (2018). Quantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damage. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 808, 1–9. https://doi.org/10.1016/j.mrfmmm.2018.01.004en_US
dc.identifier.doi10.1016/j.mrfmmm.2018.01.004en_US
dc.identifier.endpage9en_US
dc.identifier.issn0027-5107en_US
dc.identifier.issn1873-135Xen_US
dc.identifier.pmid29366947en_US
dc.identifier.scopus2-s2.0-85040666884en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.mrfmmm.2018.01.004
dc.identifier.urihttps://hdl.handle.net/20.500.12713/811
dc.identifier.volume808en_US
dc.identifier.wosWOS:000428342000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorUlukaya, Enginen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofMutation Research-Fundamental and Molecular Mechanisms of Mutagenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOxidative Dna Damageen_US
dc.subjectAntioxidanten_US
dc.subjectThymeen_US
dc.subjectLung Canceren_US
dc.subjectGc-Ms/Msen_US
dc.titleQuantification of DNA damage products by gas chromatography tandem mass spectrometry in lung cell lines and prevention effect of thyme antioxidants on oxidative induced DNA damageen_US
dc.typeArticleen_US

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