Histopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in rats

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dc.authoridErdal Karaöz / 0000-0002-9992-833Xen_US
dc.authorscopusidErdal Karaöz / 7003448087
dc.authorwosidErdal Karaöz / IXE-6677-2023
dc.contributor.authorYüksel, Serdar
dc.contributor.authorGüleç, M. Akif
dc.contributor.authorGültekin, Zeki
dc.contributor.authorÇağlar, Aysel
dc.contributor.authorBeytemur, Ozan
dc.contributor.authorAlagöz, Ender
dc.contributor.authorEker, A. Akdoğan
dc.contributor.authorSubaşı, Cansu
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned2020-08-30T20:06:54Z
dc.date.available2020-08-30T20:06:54Z
dc.date.issued2019
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: This study aimed to assess Achilles tendon repair in rats following splenectomy to simulate patients with musculoskeletal system injury who had splenectomy after spleen injury, a situation often seen in orthopedics and traumatology practice. Materials and Methods: The study included 32 male Sprague-Dawley rats (10 months old; average weight, 394.5 +/- 28.3 g). The rats were fed with standard rodent food ad libitum at 22 degrees C in a dark environment for 12 h. They were divided into two groups, namely the splenectomy (total splenectomy and Achilles tendon repair) and control groups (only Achilles tendon repair; n = 16). Four weeks after the surgery, the rats were euthanized, and their Achilles tendons were examined histopathologically, immunohistochemically, and biomechanically. Results: In the splenectomy group, proinflammatory cytokines, such as interleukin-1 beta, tumor necrosis factor-alpha, and interferon-gamma, showed significantly lower values than those in the control group (p <0.01); moreover, the levels of anti-inflammatory cytokines like vascular endothelial growth factor, transforming growth factor-beta 1, interleukin-2, interleukin-10, and hepatocyte growth factor were significantly higher than in the control group (p < 0.001). The average ultimate tensile strengths were 2.58 +/- 0.5 in the splenectomy and 2.78 +/- 0.3 in the control group (p = 0.043). The average values were 0.33 +/- 0.5 in the splenectomy and 0.44 +/- 0.1 in the control group (p = 0.021). Conclusion: Splenectomy may positively influence Achilles tendon healing through modification of the proinflammatory/anti-inflammatory ratio in favor of anti-inflammatory cytokines by causing a decrease in spleen-originated inflammatory cells.en_US
dc.description.sponsorshipBagcilar Training and Research Hospital Experimental Research and Skill Development Center (BADABEM)Istanbul Bagcilar Training & Research Hospitalen_US
dc.description.sponsorshipWe would like to thank the Bagcilar Training and Research Hospital Experimental Research and Skill Development Center (BADABEM) for their kind contributions and support. We also appreciate the assistance of Dr. B. Onur Kucukyildirim and Mr. Ali Avci from Yildiz Technical University, Mechanical Engineering Department and Advanced Materials Research Group (AMRG), for their help during the biomechanical laboratory analyses.en_US
dc.identifier.citationYuksel, S., Gulec, M. A., Gultekin, Z., Caglar, A., Beytemur, O., Alagoz, E., … Karaoz, E. (2019). Histopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in rats. CONNECTIVE TISSUE RESEARCH, 60(2), 200–208. https://doi.org/10.1080/03008207.2018.1483361en_US
dc.identifier.doi10.1080/03008207.2018.1483361en_US
dc.identifier.endpage208en_US
dc.identifier.issn0300-8207en_US
dc.identifier.issn1607-8438en_US
dc.identifier.issue2en_US
dc.identifier.pmid29860899en_US
dc.identifier.scopus2-s2.0-85048771533en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage200en_US
dc.identifier.urihttps://doi.org/10.1080/03008207.2018.1483361
dc.identifier.urihttps://hdl.handle.net/20.500.12713/648
dc.identifier.volume60en_US
dc.identifier.wosWOS:000461646700013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaraöz, Erdalen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAchilles Tendonen_US
dc.subjectCytokinesen_US
dc.subjectGrowth Factoren_US
dc.subjectRatsen_US
dc.subjectSplenectomyen_US
dc.titleHistopathological, immunohistochemical, and biomechanical effects of splenectomy on Achilles tendon healing in ratsen_US
dc.typeArticleen_US

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