Epigallocatechin-3-gallate and resveratrol attenuate hydrogen peroxide induced damage in neuronal cells

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dc.authoridcoşar, meltem/0000-0003-1658-5122
dc.authoridErdogan, Mumin Alper/0000-0003-0048-444X
dc.authoridYILMAZ, OZLEM/0000-0001-5744-0937
dc.authorwosidcoşar, meltem/AGF-1077-2022
dc.authorwosidErdogan, Mumin Alper/AAR-3140-2021
dc.authorwosidYILMAZ, OZLEM/A-1458-2019
dc.contributor.authorCosar, Meltem Yilmaz
dc.contributor.authorErdogan, Mumin Alper
dc.contributor.authorYilmaz, Ozlem
dc.date.accessioned2024-05-19T14:41:19Z
dc.date.available2024-05-19T14:41:19Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractPURPOSE: The purpose of this study is to investigate how the antioxidants resveratrol and epigallocatechin-3-gallate (EGCG) protect SH-SY5Y cells against damage caused by hydrogen peroxide (H2O2). METHODS : SH-SY5Y cells were pretreated with EGCG and resveratrol at concentrations of 0.1 pM, 1 pM, and 10 pM, individually and in various combinations. Cells were exposed to 250 pM H2O2 for 1-hour following a 24-hour pretreatment. The effects of EGCG and resveratrol on cellular survival against hydrogen peroxide toxicity were evaluated with the MTS. Caspase 3 levels were measured with caspase 3 ELISA test for evaluating survival. The clonogenic test was utilized to assess the colony forming capacity. RESULTS: The MTS test revealed that pretreatment of SH-SY5Y cells for 24 hours with EGCG and Resveratrol enhanced cellular survival against hydrogen peroxide damage at all dosages (p < 0.005). The caspase 3 ELISA test revealed that EGCG and resveratrol significantly decreased caspase 3 levels and improved cellular survival via the caspase 3 pathway (p < 0.005). The clonogenic test findings show that resveratrol and EGCG statistically boost SH-SY5Y cells' potential to form colonies (p<0.005). CONCLUSIONS: By reducing caspase 3 levels in exposure to hydrogen peroxide damage, EGCG and resveratrol promote cellular viability (Tab. 1, Fig. 3, Ref. 37). Text in PDF www.elis.sken_US
dc.description.sponsorshipEge University Scientific Research Fund [TYL-2020-21274]en_US
dc.description.sponsorshipAcknowledgements: This study was supported by grant TYL-2020-21274 from the Ege University Scientific Research Fund.en_US
dc.identifier.doi10.4149/BLL_2023_033
dc.identifier.endpage211en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue3en_US
dc.identifier.pmid36598312en_US
dc.identifier.scopus2-s2.0-85147834376en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage205en_US
dc.identifier.urihttps://doi.org10.4149/BLL_2023_033
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5093
dc.identifier.volume124en_US
dc.identifier.wosWOS:000944162900008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAepress Sroen_US
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectEpigallocatechin-3-Gallateen_US
dc.subjectResveratrolen_US
dc.subjectHydrogen Peroxideen_US
dc.subjectNeurodegenerationen_US
dc.titleEpigallocatechin-3-gallate and resveratrol attenuate hydrogen peroxide induced damage in neuronal cellsen_US
dc.typeArticleen_US

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