The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats

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dc.authoridSalim Ersözlü / 0000-0002-5383-9665
dc.authorscopusidSalim Ersözlü / 8869531800
dc.authorwosidSalim Ersözlü / JGW-4733-2023
dc.contributor.authorErsözlü, Salim
dc.contributor.authorSarısözen, Bartu
dc.contributor.authorÖzer, Özgür
dc.contributor.authorAdım, Saduman Balaban
dc.contributor.authorŞahin, Orçun
dc.date.accessioned2020-08-30T20:01:40Z
dc.date.available2020-08-30T20:01:40Z
dc.date.issued2017
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Although, glucocorticoid (GC) and calcitonin-induced changes in bone repair have been studied previously, the exact effects of these on fracture healing remain controversial. Hence, the purpose of this experimental study is to determine biochemical and histological effects of locally administrated GC and systemically administrated calcitonin on the kinetics of healing response after bone marrow ablation in rats. Methods: After having undergone marrow ablation, a steroid-treated group of rats (n = 24) received a single dose of intramedullary methylprednisolone (2 mg/kg), a calcitonin-treated group (n = 24) received intermittently administrated subcutaneous salmon calcitonin (16 IU/kg), and a control group (n = 24) received intramedullary saline (25 ?l). Results: Blood samples taken on days 1, 3, 7, 9, and 15 after ablation showed an increase in serum calcium, alkaline phosphatase (ALP), and phosphate levels in the Calcitonin and Control groups. Levels of calcium and ALP peaked on day 7 after ablation. However, an increase in phosphate levels indicated a biphasic reaction that peaked on the third and ninth day after ablation. Hypercalcemia was not observed in Steroid group because of the inhibition of osteoclastic bone resorption. In that group, the serum levels of ALP and phosphate were lower than baseline levels. The levels of urinary calcium excretion peaked 3 to 7 days after marrow ablation in the control group and 7 to 9 days after that procedure in the steroid group. Histologic evaluation showed that the rats in the control group demonstrated the expected healing period according to the histological grades and that a delay in healing occurred in the calcitonin group after day 9 because of the inhibition of osteoclastic bone resorption. All rats in the steroid group exhibited a decrease and delayed healing response. Conclusion: Total serum calcium, phosphate, and ALP levels increased after bilateral tibial bone marrow ablation and urine calcium and hydroxyproline excretion also increased as a factor of bone resorption. Subcutaneously administrated salmon calcitonin did not affect biochemical changes after marrow ablation. Single-dose intramedullary methylprednisolone inhibited extra-tibial bone resorption induced by cytokines after bone marrow ablation. © 2017, The Author(s).en_US
dc.identifier.citationErsozlu, S., Sarisozen, B., Ozer, O., Adim, S. B., & Sahin, O. (2017). The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats. Journal of Experimental Orthopaedics, 4(1), 1-9.en_US
dc.identifier.doi10.1186/s40634-017-0100-xen_US
dc.identifier.issn2197-1153en_US
dc.identifier.issue1en_US
dc.identifier.pmid28730582en_US
dc.identifier.scopus2-s2.0-85066421204en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1186/s40634-017-0100-x
dc.identifier.urihttps://hdl.handle.net/20.500.12713/349
dc.identifier.volume4en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorErsözlü, Salimen_US
dc.language.isoenen_US
dc.publisherSpringer Berlin Heidelbergen_US
dc.relation.ispartofJournal of Experimental Orthopaedicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBone Repairen_US
dc.subjectCalcitoninen_US
dc.subjectFracture Healingen_US
dc.subjectMarrow Ablationen_US
dc.subjectMethylprednisoloneen_US
dc.titleThe biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in ratsen_US
dc.typeArticleen_US

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