Effect of embelin on inhibition of cell growth and induction of apoptosis in Acanthamoeba castellanii

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dc.authoridShaikh, Mohd. Farooq/0000-0001-9865-6224
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authorwosidShaikh, Mohd. Farooq/H-6029-2019
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.contributor.authorAhmed, Usman
dc.contributor.authorOng, Seng-Kai
dc.contributor.authorKhan, Khalid Mohammed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorShaikh, Mohd Farooq
dc.contributor.authorAlawfi, Bader Saleem
dc.date.accessioned2024-05-20T14:38:45Z
dc.date.available2024-05-20T14:38:45Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAcanthamoeba castellanii is the causative agent of fatal encephalitis and blinding keratitis. Current therapies remain a challenge, hence there is a need to search for new therapeutics. Here, we tested embelin (EMB) and silver nanoparticles doped with embelin (EMB-AgNPs) against A. castellanii. Using amoebicidal assays, the results revealed that both compounds inhibited the viability of Acanthamoeba, having an IC50 of 27.16 +/- 0.63 and 13.63 +/- 1.08 mu M, respectively, while causing minimal cytotoxicity against HaCaT cells in vitro. The findings suggest that both samples induced apoptosis through the mitochondria-mediated pathway. Differentially expressed genes analysis showed that 652 genes were uniquely expressed in treated versus untreated cells, out of which 191 were significantly regulated in the negative control vs. conjugate. Combining the analysis, seven genes (ARIH1, RAP1, H3, SDR16C5, GST, SRX1, and PFN) were highlighted as the most significant (Log2 (FC) value +/- 4) for the molecular mode of action in vitro. The KEGG analysis linked most of the genes to apoptosis, the oxidative stress signaling pathway, cytochrome P450, Rap1, and the oxytocin signaling pathways. In summary, this study provides a thorough framework for developing therapeutic agents against microbial infections using EMB and EMB-AgNPs.en_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia, Monash University, Malaysia, and the H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi.; Sunway University, Malaysia, Monash University, Malaysia; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachien_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia, Monash University, Malaysia, and the H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi.en_US
dc.identifier.doi10.1007/s00203-023-03698-3
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue12en_US
dc.identifier.pmid37898989en_US
dc.identifier.scopus2-s2.0-85175265157en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org10.1007/s00203-023-03698-3
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4592
dc.identifier.volume205en_US
dc.identifier.wosWOS:001098071300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectA. Castellaniien_US
dc.subjectAnti-Amoebicen_US
dc.subjectEmbelinen_US
dc.subjectSilver Nanoparticles Coated With Embelinen_US
dc.subjectApoptosisen_US
dc.subjectTranscriptome Analysisen_US
dc.titleEffect of embelin on inhibition of cell growth and induction of apoptosis in Acanthamoeba castellaniien_US
dc.typeArticleen_US

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