Emodin and aloe-emodin, two potential molecules in regulating cell migration of skin cells through the MAP kinase pathway and affecting Caenorhabditis elegans thermotolerance

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dc.authoridKURAN, EBRU DIDEM/0000-0001-5068-0188
dc.authoridGunaydin Akyildiz, Aysenur/0000-0003-1196-9530
dc.authoridAlim Toraman, Gülbahar Özge/0000-0002-7816-7551
dc.authoridAtasoy, Sezen/0000-0001-5063-5053
dc.authorwosidKURAN, EBRU DIDEM/AEJ-4249-2022
dc.authorwosidGunaydin Akyildiz, Aysenur/AAK-1806-2021
dc.authorwosidAlim Toraman, Gülbahar Özge/AAR-8189-2021
dc.authorwosidGüleç, Meltem/M-4510-2018
dc.contributor.authorGunaydin-Akyildiz, Aysenur
dc.contributor.authorYanikoglu, Rabia Sare
dc.contributor.authorGulec, Meltem
dc.contributor.authorAlim-Toraman, Gulbahar Ozge
dc.contributor.authorKuran, Ebru Didem
dc.contributor.authorAtasoy, Sezen
dc.contributor.authorOlgun, Abdullah
dc.date.accessioned2024-05-19T14:41:13Z
dc.date.available2024-05-19T14:41:13Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackgroundEmodin and aloe-emodin are two anthraquinones having positive effects in wound healing. However, their mechanism of action of wound healing is not fully understood. The MAP kinase family, which plays an active role in wound healing, is a well-characterized large family of serine/threonine kinases and regulates processes such as proliferation, oncogenesis, differentiation, and inflammation in the cell. The aim of this study is to comparatively elucidate the mechanisms of action of emodin and aloe-emodin, which are potential agents in wound healing.MethodsThe mechanism of the effects of emodin and aloe-emodin on cell viability and cell migration was examined using the human skin fibroblast (CCD-1079Sk) cell line. The gene expression levels of the MAP kinases (JNK, P38, ERK) in the skin fibroblast cells along with a molecular docking study analyzing their interaction potential were evaluated. Furthermore, the molecules' effects on the lifespan of Caenorhabditis elegans were studied.ResultsEmodin and aloe-emodin inhibited the ATP content of the cells in a concentration dependent manner and accelerated cell migration at the lower concentrations while inhibiting cell migration in the higher concentration treatment groups. The expressions of JNK and P38 were upregulated at the low concentrations and downregulated at the higher concentrations. The molecular docking studies of the molecules gave high docking scores indicating their interaction potential with JNK and P38. C. elegans lifespan under heat stress was observed longer after 75 & mu;M emodin and was significantly reduced after 150 & mu;M aloe-emodin treatment.ConclusionAloe-emodin was found to be more potent on cell viability, cell migration, gene expression levels of the MAP kinases in healthy fibroblastic skin cells, and on the lifespan of C. elegans. This study reveals the functional effects and the biological factors that interact in the wound healing process of emodin and aloe-emodin, and give a possible treatment alternative to shorten the duration of wound care.en_US
dc.identifier.doi10.1186/s12860-023-00486-1
dc.identifier.issn2661-8850
dc.identifier.issue1en_US
dc.identifier.pmid37491200en_US
dc.identifier.scopus2-s2.0-85165650546en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org10.1186/s12860-023-00486-1
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5080
dc.identifier.volume24en_US
dc.identifier.wosWOS:001032979100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBmcen_US
dc.relation.ispartofBmc Molecular and Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectEmodinen_US
dc.subjectAloe-Emodinen_US
dc.subjectMolecular Dockingen_US
dc.subjectMap Kinaseen_US
dc.subjectJnken_US
dc.subjectP38en_US
dc.subjectErken_US
dc.subjectCen_US
dc.subjectElegansen_US
dc.subjectThermotoleranceen_US
dc.titleEmodin and aloe-emodin, two potential molecules in regulating cell migration of skin cells through the MAP kinase pathway and affecting Caenorhabditis elegans thermotoleranceen_US
dc.typeArticleen_US

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