Alpha-Mangostin and its nano-conjugates induced programmed cell death in Acanthamoeba castellanii belonging to the T4 genotype

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dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.authorwosidKhan, Naveed/KCK-0156-2024
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.contributor.authorAhmed, Usman
dc.contributor.authorOng, Seng-Kai
dc.contributor.authorTan, Kuan Onn
dc.contributor.authorKhan, Khalid Mohammed
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAlawfi, Bader Saleem
dc.date.accessioned2024-05-19T14:38:46Z
dc.date.available2024-05-19T14:38:46Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAcanthamoeba are free living amoebae that are the causative agent of keratitis and granulomatous amoebic encephalitis. Alpha-Mangostin (AMS) is a significant xanthone; that demonstrates a wide range of biological activities. Here, the anti-amoebic activity of alpha-Mangostin and its silver nano conjugates (AMS-AgNPs) were evaluated against pathogenic A. castellanii trophozoites and cysts in vitro. Amoebicidal assays showed that both AMS and AMS-AgNPs inhibited the viability of A. castellanii dose-dependently, with an IC50 of 88.5 +/- 2.04 and 20.2 +/- 2.17 mu M, respectively. Both formulations inhibited A. castellanii-mediated human keratinocyte cell cytopathogenicity. Functional assays showed that both samples caused apoptosis through the mitochondrial pathway and reduced mitochondrial membrane potential and ATP production, while increasing reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome-c reductase in the cytosol. Whole transcriptome sequencing of A. castellanii showed the expression of 826 genes, with 447 genes being up-regulated and 379 genes being down-regulated post treatment. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the majority of genes were linked to apoptosis, autophagy, RAP1, AGE-RAGE and oxytocin signalling pathways. Seven genes (PTEN, H3, ARIH1, SDR16C5, PFN, glnA GLUL, and SRX1) were identified as the most significant (Log2 (FC) value 4) for molecular mode of action in vitro. Future in vivo studies with AMS and nanoconjugates are needed to realize the clinical potential of this work.en_US
dc.description.sponsorshipAir Force Office of Scientific Research; Sunway University, Malaysia; University of Karachi, Pakistanen_US
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia, and University of Karachi, Pakistan.en_US
dc.identifier.doi10.1007/s10123-023-00450-1
dc.identifier.issn1139-6709
dc.identifier.issn1618-1905
dc.identifier.pmid38015290en_US
dc.identifier.scopus2-s2.0-85177782761en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org10.1007/s10123-023-00450-1
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4598
dc.identifier.wosWOS:001110274000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofInternational Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectAcanthamoeba Castellaniien_US
dc.subjectAlpha-Mangostinen_US
dc.subjectAnti-Amoebicen_US
dc.subjectApoptosisen_US
dc.subjectNext Generation Sequencingen_US
dc.titleAlpha-Mangostin and its nano-conjugates induced programmed cell death in Acanthamoeba castellanii belonging to the T4 genotypeen_US
dc.typeArticleen_US

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