How does infection with human papillomavirus 16 and 18 impact on dna damage and repair in cervical cells and peripheral blood?

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dc.authoridSemra Şardaş / 0000-0001-5456-8636en_US
dc.authorscopusidSemra Şardaş / 7003286974
dc.authorwosidSemra Şardaş / J-2175-2018
dc.contributor.authorÖzçağlı, Eren
dc.contributor.authorBiri, Aydan
dc.contributor.authorDinç, Bedia
dc.contributor.authorŞardaş, Semra
dc.date.accessioned2020-08-30T20:07:36Z
dc.date.available2020-08-30T20:07:36Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractHuman papillomavirus (HPV) infection is one of the most common sexually transmitted diseases worldwide and a prime cause of cervical cancer. The HPV DNA is detected in approximately 80-90% of all cervical cancers, with HPV 16 and 18 being the high risk conferring human carcinogens. DNA damage and diminished DNA repair mechanisms are potential biological surrogates of HPV infection that warrant further research in different tissues and populations. Notably, we do not know the extent to which the high risk HPV 16 and 18 differentially affect cervical cells versus other systems such as peripheral blood lymphocytes (PBLs). We evaluated DNA damage and repair in women who tested positive for HPV 16 or HPV 18 and healthy control women without HPV 16 or HPV 18 infection. We found that the DNA damage as measured by the Comet assay was markedly greater in cervical cells of women with HPV 16 (mean: 8.1 as% DNA in tail, 95% CI: 7.6-8.7) or HPV 18 infection (mean: 9.6, 95% CI: 8.9-10.2) than controls (mean: 6.7, 95% CI: 6.2-7.4) (p<0.05). By contrast, in PBLs, we did not find a significant difference in DNA damage between women with HPV 16 or 18 infection versus controls, as measured by the Comet assay or the Conventional Chromosomal Aberration analysis (p>0.05). We observed, however, the DNA repair capacity, as measured by the X-ray induced challenge (XRC) assay, was significantly impaired in PBLs from women with HPV 16 or 18 infection compared to controls (p<0.05). This is the first comparative study, to the best of our knowledge, suggesting that the cervical swab cells might be better suited than peripheral lymphocytes as biosamples for detection of HPV 16 or 18 biological effects on DNA damage. In addition, these findings suggest that the Comet assay performed only in PBLs may potentially lead to false negative diagnosis of DNA damage. Taken together, these observations contribute to development of future diagnostic innovation and precision sampling strategies for robust detection of the biological effects of HPV 16 or 18 in women. We conclude by a brief discussion of implications for HPV clinical diagnostics and precision medicine innovation.en_US
dc.identifier.citationOzcagli, E., Biri, A., Dinc, B., & Sardas, S. (2018). How Does Infection with Human Papillomavirus 16 and 18 Impact on DNA Damage and Repair in Cervical Cells and Peripheral Blood? Omics : A Journal of Integrative Biology. https://doi.org/10.1089/omi.2018.0065en_US
dc.identifier.doi10.1089/omi.2018.0065en_US
dc.identifier.endpage336en_US
dc.identifier.issn1536-2310en_US
dc.identifier.issn1557-8100en_US
dc.identifier.issue5en_US
dc.identifier.pmid29708858en_US
dc.identifier.scopus2-s2.0-85047148083en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage332en_US
dc.identifier.urihttps://doi.org/10.1089/omi.2018.0065
dc.identifier.urihttps://hdl.handle.net/20.500.12713/797
dc.identifier.volume22en_US
dc.identifier.wosWOS:000431104700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞardaş, Semraen_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofOmics-A Journal of Integrative Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHuman Papillomavirusen_US
dc.subjectCervical Canceren_US
dc.subjectDiagnosticsen_US
dc.subjectBiomarkersen_US
dc.subjectPrecision Medicineen_US
dc.titleHow does infection with human papillomavirus 16 and 18 impact on dna damage and repair in cervical cells and peripheral blood?en_US
dc.typeArticleen_US

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