Mesoporous silica@chitosan@gold nanoparticles as “on/off” optical biosensor and pH-sensitive theranostic platform against cancer

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dc.authoridAli Zarrabi / 0000-0003-0391-1769en_US
dc.authorscopusidAli Zarrabi / 23483174100
dc.authorwosidAli Zarrabi / 0000-0003-0391-1769
dc.contributor.authorEsmaeili, Y.
dc.contributor.authorKhavani, M.
dc.contributor.authorBigham, A.
dc.contributor.authorSanati, A.
dc.contributor.authorBidram, E.
dc.contributor.authorZarrabi, A.
dc.date.accessioned2022-01-26T12:08:04Z
dc.date.available2022-01-26T12:08:04Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.description.abstractA cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells. Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt). The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy. Besides, the interaction between aptamer and curcumin was evaluated using full atomistic molecular dynamics simulations. The mechanism of curcumin release was likewise investigated through different kinetic models. Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry. It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an “on/off” optical biosensor. On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells. Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence (“On” state). Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting. Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery. © 2022en_US
dc.identifier.citationEsmaeili, Y., Khavani, M., Bigham, A., Sanati, A., Bidram, E., Shariati, L., . . . Rafienia, M. (2022). Mesoporous silica@chitosan@gold nanoparticles as “on/off” optical biosensor and pH-sensitive theranostic platform against cancer. International Journal of Biological Macromolecules, 202, 241-255. doi:10.1016/j.ijbiomac.2022.01.063en_US
dc.identifier.doi10.1016/j.ijbiomac.2022.01.063en_US
dc.identifier.endpage255en_US
dc.identifier.issn0141-8130en_US
dc.identifier.pmid35041881en_US
dc.identifier.scopus2-s2.0-85122934726en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage241en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2022.01.063
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2421
dc.identifier.volume202en_US
dc.identifier.wosWOS:000783018500004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZarrabi, Ali
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCancer Nanotheranosticen_US
dc.subjectChitosan Nanoparticlesen_US
dc.subjectMesoporous Silica Nanoparticlesen_US
dc.subjectOn/off Fluorescence Imagingen_US
dc.subjectTargeted Curcumin Deliveryen_US
dc.titleMesoporous silica@chitosan@gold nanoparticles as “on/off” optical biosensor and pH-sensitive theranostic platform against canceren_US
dc.typeArticleen_US

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