Evaluation of the optimal sampling approach for HPV genotyping in circumcised heterosexual men with genital warts

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dc.authoridBARUT, ZERRIN/0000-0002-6289-5562
dc.authorwosidOZEN, HATICE NEVGUN/ADO-8721-2022
dc.authorwosidBarut, Zerrin/IZQ-2308-2023
dc.contributor.authorSarier, Mehmet
dc.contributor.authorSepin, Nevgun
dc.contributor.authorEmek, Mestan
dc.contributor.authorKonuk, Elcin Yenidunya
dc.contributor.authorKaplan, Tugrul
dc.contributor.authorYuksel, Burcu Aykan
dc.contributor.authorBarut, Zerrin
dc.date.accessioned2024-05-19T14:46:11Z
dc.date.available2024-05-19T14:46:11Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractBackground: Human papillomavirus (HPV) causes a serious socioeconomic burden globally. However, there is currently no consensus on the optimal sampling method for HPVDNA genotyping in circumcised heterosexual men. This study aimed to determine the diagnostic efficacy of 6 different anatomic sampling sites in HPV DNA polymerase chain reaction (PCR) testing of circumcised heterosexual men with genital warts.Methods: The study included circumcised heterosexual men who presented to our clinic with complaints of genital warts. Swab samples were obtained from the penile shaft (PS), scrotum, coronal sulcus (CS), and external urethral meatus (EUM). First-void urine (FVU) and genital wart biopsy (GWB) were also tested for HPV DNA by PCR.Results: A total of 32 patients (mean age: 36.9 +/- 6.9 years) were included. None of the six samples studied was sufficient on its own to reveal all HPV types detected in a patient. When the samples were analyzed individually, GWB detected an average of 49.5% of total HPV types in a patient. This rate was 50.5% for PS, 40.4% for CS, 31.6% for scrotum, 26.3% for EUM, and 15.8% for FVU samples. The detection rate increased to 75.8% with combined testing of GWB and PS samples, 83.2% with GWB/PS/CS, 90.5% with GWB/PS/CS/scrotum, and 98.9% with GWB/PS/CS/scrotum/EUM samples.Conclusion: No single anatomic region or sample type can detect all HPV types present in circumcised heterosexual men by PCR assay. The detection rate approaches 99% when wart biopsy is combined with swab sampling of the penile shaft, coronal sulcus, scrotum, and external urethral meatus.en_US
dc.identifier.doi10.1016/j.jiac.2023.01.017
dc.identifier.endpage480en_US
dc.identifier.issn1341-321X
dc.identifier.issn1437-7780
dc.identifier.issue5en_US
dc.identifier.pmid36731776en_US
dc.identifier.scopus2-s2.0-85147299668en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage475en_US
dc.identifier.urihttps://doi.org10.1016/j.jiac.2023.01.017
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5463
dc.identifier.volume29en_US
dc.identifier.wosWOS:000972116700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Infection and Chemotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectPcren_US
dc.subjectHpven_US
dc.subjectGenital Warten_US
dc.subjectSamplingen_US
dc.subjectCircumcisionen_US
dc.titleEvaluation of the optimal sampling approach for HPV genotyping in circumcised heterosexual men with genital wartsen_US
dc.typeArticleen_US

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