Expression analysis of lncRNA AK370814 involved in the barley vitamin B6 salvage pathway under salinity

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dc.authoridNermin Gözükırmızı / 0000-0002-7129-3045
dc.authoridElif Karlık / 0000-0003-0669-2725
dc.authorscopusidNermin Gözükırmızı / 6701520637
dc.authorscopusidElif Karlık / 56926577900
dc.authorwosidNermin Gözükırmızı / M-1419-2013
dc.authorwosidElif Karlık / N-6348-2018
dc.contributor.authorKarlık, Elif
dc.contributor.authorGözükırmızı, Nermin
dc.date.accessioned2020-08-30T20:07:14Z
dc.date.available2020-08-30T20:07:14Z
dc.date.issued2018
dc.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Eczane Hizmetleri Bölümüen_US
dc.description.abstractLong non-coding RNAs (lncRNAs), which are longer than > 200 nt, perform various functions in a variety of important biological processes. The aim of this study is the investigation of relative expression levels of AK372815 putative pyridoxal reductase (PLR) gene and sense lncRNA AK370814 on four barley genotypes (Hasat, Beysehir 99, Konevi 98 and Tarm 92) in response to 150 mM salinity application during 3 days post-germination. Seeds were placed randomly in petri dishes containing (a) only H2O (control), (b) 150 mM NaCl, for 72 h. RNA isolation was carried out using TriPureA (R) reagent from 150 mM salt-treated root and shoot samples. Relative expression levels of AK372815 PLR and sense lncRNA AK370814 were determined by qPCR. Results demonstrated that salinity affected the expression levels of both AK372815 PLR gene and sense lncRNA AK370814 during germination. Although expression levels of AK372815 PLR tended to be down-regulated under salinity, expression levels of sense lncRNA AK370814 were up-regulated. Another goal of this study is improvement of alternative approach to NGS technologies for determination of relative expression levels of sense lncRNAs under particular circumstances. This is the first report that demonstrates a relationship between lncRNA and vitamin B6 salvage pathway.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul UniversityIstanbul University [FDK-2016-23086]en_US
dc.description.sponsorshipThis work was supported by Scientific Research Projects Coordination Unit of Istanbul University. Project Number: FDK-2016-23086. Also, the authors thank Dr. Stuart James Lucas for his kind revision and Dr. Cem Horozoglu (Gelisim University) for helpful suggestions.en_US
dc.identifier.citationKarlik, E., & Gozukirmizi, N. (2018). Expression analysis of lncRNA AK370814 involved in the barley vitamin B6 salvage pathway under salinity. Molecular Biology Reports, 45(6), 1597–1609. https://doi.org/10.1007/s11033-018-4289-2en_US
dc.identifier.doi10.1007/s11033-018-4289-2en_US
dc.identifier.endpage1609en_US
dc.identifier.issn0301-4851en_US
dc.identifier.issn1573-4978en_US
dc.identifier.issue6en_US
dc.identifier.pmid30298351en_US
dc.identifier.scopus2-s2.0-85055045770en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1597en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-018-4289-2
dc.identifier.urihttps://hdl.handle.net/20.500.12713/723
dc.identifier.volume45en_US
dc.identifier.wosWOS:000452543200003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarlık, Elifen_US
dc.institutionauthorGözükırmızı, Nerminen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLong Non-Coding Rnasen_US
dc.subjectVitamin B6en_US
dc.subjectVitamin B6 Salvage Pathwayen_US
dc.subjectSalinityen_US
dc.subjectBarleyen_US
dc.titleExpression analysis of lncRNA AK370814 involved in the barley vitamin B6 salvage pathway under salinityen_US
dc.typeArticleen_US

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